Product Info Summary
SKU: | M00631 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-SUMO-1 Monoclonal Antibody
SKU/Catalog Number
M00631
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-SUMO-1 Monoclonal Antibody (Catalog # M00631). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-SUMO-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00631)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM571
Isotype
IgG1, kappa
Immunogen
Recombinant human SUMO1 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00631 is reactive to SUMO1 in Human
Applications
M00631 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
11.557kDa
Background of SUMO1
This monoclonal antibody is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Anti-SUMO-1 Monoclonal Antibody (SPM571)
Protein Target Info & Infographic
Gene/Protein Information For SUMO1 (Source: Uniprot.org, NCBI)
Gene Name
SUMO1
Full Name
Small ubiquitin-related modifier 1
Weight
11.557kDa
Superfamily
ubiquitin family
Alternative Names
DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP1SMT3CSMT3H3OFC10UBL1PIC1; PIC1; SENP2; Sentrin; small ubiquitin-related modifier 1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 1 (yeast); SMT3; SMT3C; SMT3H3; SUMO1; SUMO-1; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1 SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 small ubiquitin like modifier 1 small ubiquitin-related modifier 1|GAP modifying protein 1|SMT3 homolog 3|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-homology domain protein PIC1|ubiquitin-like protein SMT3C|ubiquitin-like protein UBL1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUMO1, check out the SUMO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SUMO-1 Monoclonal Antibody (M00631)
Hello CJ!
M00631 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
3 Customer Q&As for Anti-SUMO-1 Monoclonal Antibody
Question
We have observed staining in human colon adenocarcinoma. Do you have any suggestions? Is anti-SUMO-1 Monoclonal antibody supposed to stain colon adenocarcinoma positively?
P. Zhang
Verified customer
Asked: 2020-03-18
Answer
From literature colon adenocarcinoma does express SUMO1. From Uniprot.org, SUMO1 is expressed in caudate nucleus, brain, brain placenta, placenta, colon adenocarcinoma, amygdala, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-18
Question
We are currently using anti-SUMO-1 Monoclonal antibody M00631 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The anti-SUMO-1 Monoclonal antibody (M00631) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-23
Question
We were satisfied with the WB result of your anti-SUMO-1 Monoclonal antibody. However we have seen positive staining in placenta nucleus membrane. nucleus speckle. using this antibody. Is that expected? Could you tell me where is SUMO1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
Based on literature, placenta does express SUMO1. Generally SUMO1 expresses in nucleus membrane. nucleus speckle. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-09-19