Anti-SUMO-1 Monoclonal Antibody

SUMO1 antibody

Boster Bio Anti-SUMO-1 Monoclonal Antibody (Catalog # M00631). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00631
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

Customers Who Bought This Also Bought

Product Name

Anti-SUMO-1 Monoclonal Antibody

View all SUMO1 Antibodies

SKU/Catalog Number

M00631

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-SUMO-1 Monoclonal Antibody (Catalog # M00631). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-SUMO-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00631)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM571

Isotype

IgG1, kappa

Immunogen

Recombinant human SUMO1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00631 is reactive to SUMO1 in Human

Applications

M00631 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

11.557kDa

Background of SUMO1

This monoclonal antibody is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For SUMO1 (Source: Uniprot.org, NCBI)

Gene Name

SUMO1

Full Name

Small ubiquitin-related modifier 1

Weight

11.557kDa

Superfamily

ubiquitin family

Alternative Names

DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP1SMT3CSMT3H3OFC10UBL1PIC1; PIC1; SENP2; Sentrin; small ubiquitin-related modifier 1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 1 (yeast); SMT3; SMT3C; SMT3H3; SUMO1; SUMO-1; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1 SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 small ubiquitin like modifier 1 small ubiquitin-related modifier 1|GAP modifying protein 1|SMT3 homolog 3|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-homology domain protein PIC1|ubiquitin-like protein SMT3C|ubiquitin-like protein UBL1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SUMO1, check out the SUMO1 Infographic

SUMO1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SUMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00631 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes

Have you used Anti-SUMO-1 Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-SUMO-1 Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Anti-SUMO-1 Monoclonal Antibody

Question

We have observed staining in human colon adenocarcinoma. Do you have any suggestions? Is anti-SUMO-1 Monoclonal antibody supposed to stain colon adenocarcinoma positively?

P. Zhang

Verified customer

Asked: 2020-03-18

Answer

From literature colon adenocarcinoma does express SUMO1. From Uniprot.org, SUMO1 is expressed in caudate nucleus, brain, brain placenta, placenta, colon adenocarcinoma, amygdala, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-18

Question

We are currently using anti-SUMO-1 Monoclonal antibody M00631 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

The anti-SUMO-1 Monoclonal antibody (M00631) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-23

Question

We were satisfied with the WB result of your anti-SUMO-1 Monoclonal antibody. However we have seen positive staining in placenta nucleus membrane. nucleus speckle. using this antibody. Is that expected? Could you tell me where is SUMO1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-09-19

Answer

Based on literature, placenta does express SUMO1. Generally SUMO1 expresses in nucleus membrane. nucleus speckle. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-09-19

Order DetailsPrice
M00631-100ug

100 μg

$468

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M00631
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.