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SKU:PA1474
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-survivin Antibody
SKU/Catalog Number PA1474
Description Rabbit IgG polyclonal antibody for Baculoviral IAP repeat-containing protein 5(BIRC5) detection. Tested with WB in Human.
Cite This Product Anti-survivin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1474)
Replacement Item This antibody may replace the following items: sc-163274|sc-163273 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Canine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human survivin(121-142aa KKEFEETAKKVRRAIEQLAAMD).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name BIRC5
Protein Name Baculoviral IAP repeat-containing protein 5
Molecular Weight 16389 MW
Protein Function Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. .
Tissue Specificity Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. .
Sequence Similarities Belongs to the IAP family.
Subcellular Localization Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export.
Uniprot ID O15392
Alternative Names Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin;BIRC5;API4, IAP4;
Research Areas |cell biology|apoptosis|intracellular|survivin / iaps| epigenetics and nuclear signaling|chromosome structure|chromosome|scaffold proteins|cell cycle|nuclear|transcription|transcription factors| cell biology|cell division|other cell division antibodies| cancer|invasion/microenvironment| kits/ lysates/ other|elisa kits|apoptosis marker and proteins elisa kits|neuroscience target elisa kits|cell death|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Baculoviral IAP repeat-containing protein 5

Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis(IAP) family. The survivin gene contains 4 exons. The survivin gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.

Anti-survivin Antibody Images

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Anti-survivin Antibody
Anti-survivin antibody, PA1474, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: CEM Cell Lysate
Lane 4: COLO320 Cell Lysate
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Publications

Shan Sj, Zhang N, Geng Sl, Zhou Z, Chen X, Nie T, Guo Z, Li C, Liu Q, Guo Y, Wei H, Chen Hd. J Cutan Pathol. 2010 Jun;37(6):635-40. Doi: 10.1111/J.1600-0560.2009.01437.X. Epub 2009 Sep 24. Expression Of Survivin And Human Telomerase Reverse Transc...
Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X. Biochem Biophys Res Commun. 2013 May 17;434(4):809-14. Doi: 10.1016/J.Bbrc.2013.04.018. Epub 2013 Apr 20. Aspirin Overcomes Navitoclax-Resistance In Hepatocellular Carcinoma Cells Through S...
Zhang B, Lu Z, Hou Y, Hu J, Wang C. Tumour Biol. 2014 Jun;35(6):5401-7. Doi: 10.1007/S13277-014-1704-8. Epub 2014 Feb 12. The Effects Of Stat3 And Survivin Silencing On The Growth Of Human Bladder Carcinoma Cells.
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...
Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.