|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-Synaptophysin Antibody (Monoclonal, SVP-38)|
|Description||Mouse IgG monoclonal antibody for Synaptophysin, synaptophysin (SYP) detection. Tested with WB, IHC-P, IHC-F in Human;Rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Synaptophysin Antibody (Monoclonal, SVP-38) SVP-38 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1091)|
|Replacement Item||This antibody may replace the following items: sc-55507|sc-58304|sc-12737|sc-7569|sc-9116|sc-17750|sc-7568|sc-365447|sc-365488|sc-58301|sc-136271 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Rat retina synaptosome.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||33311 MW|
|Protein Function||Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). .|
|Tissue Specificity||Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.|
|Sequence Similarities||Belongs to the synaptophysin/synaptobrevin family.|
|Subcellular Localization||Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.|
|Alternative Names||Synaptophysin;Major synaptic vesicle protein p38;Syp;|
Background for Synaptophysin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Synaptophysin Antibody (Monoclonal, SVP-38) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, rat, -
Western blot, 0.5-1μg/ml, Human, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Synaptophysin Antibody (Monoclonal, SVP-38) Images
Click the images to enlarge.
WB: Rat Brain Tissue Lysate
IHC(P): Rat Brain Tissue
IHC(P): Human Glioma Tissue
IHC(P): Human meningeoma Tissue