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SKU:MA1091
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:SVP-38
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-Synaptophysin Antibody (Monoclonal, SVP-38)
SKU/Catalog Number MA1091
Description Mouse IgG monoclonal antibody for Synaptophysin, synaptophysin (SYP) detection. Tested with WB, IHC-P, IHC-F in Human;Rat. No cross reactivity with other proteins.
Cite This Product Anti-Synaptophysin Antibody (Monoclonal, SVP-38) SVP-38 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1091)
Replacement Item This antibody may replace the following items: sc-55507|sc-58304|sc-12737|sc-7569|sc-9116|sc-17750|sc-7568|sc-365447|sc-365488|sc-58301|sc-136271 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Rat
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Rat retina synaptosome.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number SVP-38
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Syp
Protein Name Synaptophysin
Molecular Weight 33311 MW
Protein Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). .
Tissue Specificity Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Sequence Similarities Belongs to the synaptophysin/synaptobrevin family.
Subcellular Localization Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Uniprot ID P07825
Alternative Names Synaptophysin;Major synaptic vesicle protein p38;Syp;
Research Areas SYP|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Synaptophysin

Synaptophysin(SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al.(1990) concluded that the gene has 7 exons distributed over about 20 kb. SYP mapped the SYP locus to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.

Anti-Synaptophysin Antibody (Monoclonal, SVP-38) Images

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Anti-Synaptophysin Antibody (Monoclonal, SVP-38)
Anti-Synaptophysin antibody (monoclonal), MA1091, Western blotting
WB: Rat Brain Tissue Lysate
Anti-Synaptophysin Antibody (Monoclonal, SVP-38)
Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P)
IHC(P): Rat Brain Tissue
Anti-Synaptophysin Antibody (Monoclonal, SVP-38)
Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P)
IHC(P): Human Glioma Tissue
Anti-Synaptophysin Antibody (Monoclonal, SVP-38)
Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P)
IHC(P): Human meningeoma Tissue
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Publications

Li Xh, Xin X, Wang Y, Wu Jz, Jin Zd, Ma Ln, Nie Cj, Xiao X, Hu Y, Jin Mw. J Alzheimers Dis. 2013;34(3):755-67. Doi: 10.3233/Jad-122017. Pentamethylquercetin Protects Against Diabetes-Related Cognitive Deficits In Diabetic Goto-Kakizaki Rats.
Liu Y, Liang Z, Liu J, Zou W, Li X, Wang Y, An L. Neural Regen Res. 2013 Oct 15;8(29):2725-33. Doi: 10.3969/J.Issn.1673-5374.2013.29.004. Downregulation Of Caveolin-1 Contributes To The Synaptic Plasticity Deficit In The Hippocampus Of Aged Rats.
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Autop...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to synaptophysin antibody, syp antibody