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SKU:PA1896
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Syndecan 2 Antibody
SKU/Catalog Number PA1896
Description Rabbit IgG polyclonal antibody for Syndecan-2(SDC2) detection. Tested with WB, IHC-P, IHC-F in Human.
Cite This Product Anti-Syndecan 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1896)
Replacement Item This antibody may replace the following items: sc-27493|sc-367285|sc-27494 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Syndecan 2(105-122aa EETDKEKVHLSDSERKMD).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SDC2
Protein Name Syndecan-2
Molecular Weight 22160 MW
Protein Function Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis (By similarity). .
Sequence Similarities Belongs to the syndecan proteoglycan family.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P34741
Alternative Names Syndecan-2;SYND2;Fibroglycan;Heparan sulfate proteoglycan core protein;HSPG;CD362;SDC2;HSPG1;
Research Areas |cardiovascular|angiogenesis|growth factors|vegf| neuroscience|cell adhesion proteins|ecm proteins| cardiovascular|angiogenic factors| signal transduction|cytoskeleton / ecm|hspgs|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Syndecan-2

SDC2(Syndecan-2) also known as SYND2, HSPG1, HSPG, and Fibroglycan, is a protein that in humans is encoded by the SDC2 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. By Southern hybridization to a panel of human-mouse somatic cell hybrid DNA and by in situ hybridization, Marynen et al.(1989) showed that the heparan sulfate proteoglycan core protein maps to 8q22-q24.(Heparan sulfate proteoglycan of basement membrane appears to be encoded by a gene on human chromosome 1.Bobardt et al.(2003) demonstrated that syndecans, including SDC2, can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparan sulfate chains. Flow cytometric analysis demonstrated SDC expression on endothelial cells. HIV bound to SDC on endothelial cell lines maintained its infectivity for at least 1 week, compared with less than 1 day for unbound virus. Bobardt et al.(2003) suggested that SDC-rich endothelial cells lining the vasculature can provide a microenvironment that boosts HIV replication in T cells.

Anti-Syndecan 2 Antibody Images

Click the images to enlarge.

Anti-Syndecan 2 Antibody
Anti-Syndecan 2 antibody, PA1896, IHC(P)
IHC(P): Human Liver Cancer Tissue
Anti-Syndecan 2 Antibody
Anti-Syndecan 2 antibody, PA1896, Western blotting
Lane 1: HEPG Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: SKOV Cell ysate
Lane 5: JURKAT Cell Lysate
Anti-Syndecan 2 Antibody
Anti-Syndecan 2 antibody, PA1896, IHC(F)
IHC(F): Human Placenta Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Is this antibody directed against an extra- or intra-cellular epitope of the protein?
A: The antibody is directed against an extracellular epitope of the protein.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to hspg antibody, syndecan-2 antibody