Anti-T2R8 TAS2R8 Antibody

TAS2R8 antibody

Boster Bio Anti-T2R8 TAS2R8 Antibody catalog # A12428. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A12428
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-T2R8 TAS2R8 Antibody

View all TAS2R8 Antibodies

SKU/Catalog Number

A12428

Size

100μl

Form

Liquid

Description

Boster Bio Anti-T2R8 TAS2R8 Antibody catalog # A12428. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-T2R8 TAS2R8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12428)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

6E2

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human T2R8. at AA rangle: 20-100

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12428 is reactive to TAS2R8 in Human

Applications

A12428 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

35.877kDa

Background of TAS2R8

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For TAS2R8 (Source: Uniprot.org, NCBI)

Gene Name

TAS2R8

Full Name

Taste receptor type 2 member 8

Weight

35.877kDa

Superfamily

G-protein coupled receptor T2R family

Alternative Names

Taste receptor type 2 member 8 TAS2R8 T2R8, TRB5 taste 2 receptor member 8 taste receptor type 2 member 8|taste receptor, family B, member 5|taste receptor, type 2, member 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TAS2R8, check out the TAS2R8 Infographic

TAS2R8 infographic

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In this infographic, you will see the following information for TAS2R8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-T2R8 TAS2R8 Antibody

Question

We are currently using anti-T2R8 antibody A12428 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

The anti-T2R8 antibody (A12428) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-13

Question

Does A12428 anti-T2R8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-01-10

Answer

You can see on the product datasheet, A12428 anti-T2R8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-10

Question

My question regarding product A12428, anti-T2R8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-06

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12428 anti-T2R8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-06

Order DetailsPrice
A12428

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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