|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-TAB2 Antibody|
|Description||Rabbit IgG polyclonal antibody for TGF-beta-activated kinase 1/MAP3K7-binding protein 2(TAB2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-TAB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2289)|
|Replacement Item||This antibody may replace the following items: sc-11851|sc-20756|sc-11850|sc-398188 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TAB2(487-506aa LTNLLNHPDHYVETENIQHL), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||TGF-beta-activated kinase 1/MAP3K7-binding protein 2|
|Molecular Weight||76494 MW|
|Protein Function||Adapter linking MAP3K7/TAK1 and TRAF6. Promotes MAP3K7 activation in the IL1 signaling pathway. The binding of 'Lys-63'- linked polyubiquitin chains to TAB2 promotes autophosphorylation of MAP3K7 at 'Thr-187'. Involved in heart development. .|
|Tissue Specificity||Widely expressed. In the embryo, expressed in the ventricular trabeculae, endothelial cells of the conotruncal cushions of the outflow tract and in the endothelial cells lining the developing aortic valves. .|
|Sequence Similarities||Contains 1 CUE domain.|
|Subcellular Localization||Membrane ; Peripheral membrane protein . Cytoplasm, cytosol . Following IL1 stimulation, translocation occurs from the membrane to cytosol.|
|Alternative Names||TGF-beta-activated kinase 1 and MAP3K7-binding protein 2;Mitogen-activated protein kinase kinase kinase 7-interacting protein 2;TAK1-binding protein 2;TAB-2;TGF-beta-activated kinase 1-binding protein 2;TAB2;KIAA0733, MAP3K7IP2;|
|Research Areas|||signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway| immunology|innate immunity|tlr signaling||
Background for TGF-beta-activated kinase 1/MAP3K7-binding protein 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TAB2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TAB2 Antibody Images
Click the images to enlarge.
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
All lanes: Anti-TAB2(PA2289) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 40ug
Lane 2: Mouse Testis Tissue Lysate at 40ug
Lane 3: Mouse Spleen Tissue Lysate at 40ug
Predicted bind size: 76KD
Observed bind size: 76KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,