Anti-TAF4 Antibody

TAF4 antibody

Boster Bio Anti-TAF4 Antibody (Catalog # A05511). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05511
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-TAF4 Antibody

View all TAF4 Antibodies

SKU/Catalog Number

A05511

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TAF4 Antibody (Catalog # A05511). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TAF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05511)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TAF4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05511 is reactive to TAF4 in Human, Mouse

Applications

A05511 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

110.114kDa

Background of TAF4

Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.

Mengus G., Genes Dev. 11:1381-1395(1997).
Deloukas P., Nature 414:865-871(2001).
Tanese N., Proc. Natl. Acad. Sci. U.S.A. 93:13611-13616(1996).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For TAF4 (Source: Uniprot.org, NCBI)

Gene Name

TAF4

Full Name

Transcription initiation factor TFIID subunit 4

Weight

110.114kDa

Superfamily

TAF4 family

Alternative Names

FLJ41943; TAF(II)130; TAF(II)135; TAF2C1TBP-associated factor 4; TAF2CRNA polymerase II TBP-associated factor subunit C; TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF4A; TAFII130TAFII-130; TAFII135TAFII-135; TATA box binding protein (TBP)-associated factor, 135kD; TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD; Transcription initiation factor TFIID 130 kDa subunit; transcription initiation factor TFIID 135 kD subunit; transcription initiation factor TFIID subunit 4 TAF4 TAF(II)130, TAF(II)135, TAF2C, TAF2C1A, TAFII-130, TAFII-135, TAFII130, TAFII135, TAF4 TATA-box binding protein associated factor 4 transcription initiation factor TFIID subunit 4|RNA polymerase II TBP-associated factor subunit C|TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa|TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD|TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD|TBP-associated factor 4|transcription initiation factor TFIID 130 kDa subunit|transcription initiation factor TFIID 135 kD subunit|transcription initiation factor TFIID 135 kDa subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TAF4, check out the TAF4 Infographic

TAF4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TAF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-TAF4 Antibody

Question

Please see the WB image, lot number and protocol we used for secondary oocyte using anti-TAF4 antibody A05511. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-12

Question

Does anti-TAF4 antibody A05511 work for WB with secondary oocyte?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

According to the expression profile of secondary oocyte, TAF4 is highly expressed in secondary oocyte. So, it is likely that anti-TAF4 antibody A05511 will work for WB with secondary oocyte.

Boster Scientific Support

Answered: 2019-07-05

Question

My question regarding product A05511, anti-TAF4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Z. Johnson

Verified customer

Asked: 2015-01-23

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05511 anti-TAF4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-01-23

Question

We are currently using anti-TAF4 antibody A05511 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?

P. Huang

Verified customer

Asked: 2014-04-09

Answer

The anti-TAF4 antibody (A05511) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-09

Order DetailsPrice
A05511-100uL

100uL

$360

Free Secondary Antibody

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A05511
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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