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SKU:MA1092
Clonality:Monoclonal
Clone Number:8d4
Application:WB
Price: $200.00
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Overview

Product Name Anti-Talin Antibody (Monoclonal, 8d4)
Description Mouse IgG monoclonal antibody for Talin, talin 1; talin 2 (TLN1; TLN2) detection. Tested with WB in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Talin Antibody (Monoclonal, 8d4) 8d4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1092)
Replacement Item This antibody may replace the following items: sc-20936|sc-56917|sc-57026|sc-377530|sc-53335|sc-53334|sc-21089-R from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Mouse, Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Purified chicken gizzard talin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number 8d4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Tln2
Protein Name Talin-1; Talin-2
Molecular Weight 271684 MW
Protein Function TLN1: Probably involved in connections of major cytoskeletalstructures to the plasma membrane. High molecular weightcytoskeletal protein concentrated at regions of cell-substratumcontact and, in lymphocytes, at cell-cell contacts (Bysimilarity).

TLN2: As a major component of focal adhesion plaques thatlinks integrin to the actin cytoskeleton, may play an importantrole in cell adhesion. Recruits PIP5K1C to focal adhesion plaquesand strongly activates its kinase activity (By similarity).
Sequence Similarities Contains 1 FERM domain.
Subcellular Localization TLN1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface Cell junction, focaladhesion Note: Colocalizes with LAYN at the membraneruffles. Localized preferentially in focal adhesions thanfibrillar adhesions (By similarity).
Tln1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface
TLN2: Cell junction, focal adhesion. Celljunction, synapse. Cell membrane; Peripheral membrane protein;Cytoplasmic side. Cytoplasm, cytoskeleton. Note: Focal adhesionplaques and synapses.
Tln2: Cell junction, focal adhesion Cell junction, synapse Cell membrane Cytoplasm, cytoskeleton Note: Focaladhesion plaques and synapses.
Uniprot ID D3ZA84
Alternative Names Protein Tln2 ;Tln2 ;
Research Areas signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|talin / moesin|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Talin-1; Talin-2

Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix(ECM) receptors. It has a mass of 270 kD and shares 23% N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to talin 1 antibody, talin antibody, talin-1 antibody