Data & Images
|Product Name||Anti-TBP Antibody|
|Description||Rabbit IgG polyclonal antibody for TATA-box-binding protein(TBP) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-TBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1534)|
|Replacement Item||This antibody may replace the following items: sc-26142|sc-26141|sc-33736|sc-74596|sc-74595|sc-26143 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human TBP(227-246aa EEQSRLAARKYARVVQKLGF), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||TATA-box-binding protein|
|Molecular Weight||37698 MW|
|Protein Function||General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. .|
|Tissue Specificity||Widely expressed, with levels highest in the testis and ovary. .|
|Alternative Names||TATA-box-binding protein;TATA sequence-binding protein;TATA-binding factor;TATA-box factor;Transcription initiation factor TFIID TBP subunit;TBP;GTF2D1, TF2D, TFIID;|
|Research Areas|||epigenetics and nuclear signaling|transcription|polymerase associated factors|pol ii transcription|tfiid| epigenetics and nuclear signaling|tafs| isotype/loading controls|tbp - nuclear|preinitiation complex|chip'ing antibodies||
Background for TATA-box-binding protein
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TBP Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TBP Antibody Images
Click the images to enlarge.
Lane 1: MCF-7 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: MM231 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,