Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody

TATA binding protein TBP antibody

Boster Bio Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody catalog # M00302. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00302
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody

View all TATA binding protein TBP Antibodies

SKU/Catalog Number

M00302

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody catalog # M00302. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00302)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DOA-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TBP

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00302 is reactive to TBP in Human, Mouse, Rat

Applications

M00302 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

62 kDa

Calculated molecular weight

37.698kDa

Background of TATA binding protein TBP

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For TBP (Source: Uniprot.org, NCBI)

Gene Name

TBP

Full Name

TATA-box-binding protein

Weight

37.698kDa

Superfamily

TBP family

Alternative Names

GTF2D; GTF2D1; HDL4; MGC126054; MGC126055; SCA17; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TF2D; TFIIDMGC117320; Transcription initiation factor TFIID TBP subunit TBP GTF2D, GTF2D1, HDL4, SCA17, TFIID TATA-box binding protein TATA-box-binding protein|TATA sequence-binding protein|TATA-box binding protein N-terminal domain|TATA-box factor|transcription initiation factor TFIID TBP subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TBP, check out the TBP Infographic

TBP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody

Question

We are currently using anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody M00302 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

The anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody (M00302) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-25

Question

We have seen staining in rat fibroblast. Any tips? Is anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody supposed to stain fibroblast positively?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

According to literature fibroblast does express TBP. According to Uniprot.org, TBP is expressed in forebrain, fibroblast, uterus, among other tissues. Regarding which tissues have TBP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2194289
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-07-10

Question

We are interested in using your anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody for transcription elongation from rna polymerase i promoter studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-06-28

Answer

We appreciate your inquiry. This M00302 anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody is tested on k562 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-06-28

Question

My colleagues were well pleased with the WB result of your anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody. However we have seen positive staining in forebrain nucleus. using this antibody. Is that expected? Could you tell me where is TBP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-11-21

Answer

According to literature, forebrain does express TBP. Generally TBP expresses in nucleus. Regarding which tissues have TBP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2194289
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-11-21

Order DetailsPrice
M00302

100μL

$370
M00302-30ul

30μL sample

$99
M00302-carrier-free

Carrier Free

$370

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