Product Info Summary
SKU: | M00431 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TCF7L2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00431
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TCF7L2 Rabbit Monoclonal Antibody catalog # M00431. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCF7L2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00431)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FIB-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TCF7L2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00431 is reactive to TCF7L2 in Human, Mouse, Rat
Applications
M00431 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
160-180 kDa
Calculated molecular weight
67.919kDa
Background of TCF7L2
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TCF7L2 expression in Jurkat cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colonic cancer, using TCF7L2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TCF7L2 (Source: Uniprot.org, NCBI)
Gene Name
TCF7L2
Full Name
Transcription factor 7-like 2
Weight
67.919kDa
Superfamily
TCF/LEF family
Alternative Names
HMG box transcription factor 4; hTCF-4; T-cell factor 4; T-cell factor-4 variant A; T-cell factor-4 variant B; T-cell factor-4 variant C; T-cell factor-4 variant D; T-cell factor-4 variant E; T-cell factor-4 variant H; T-cell factor-4 variant I; T-cell factor-4 variant J; T-cell factor-4 variant K; T-cell factor-4 variant L; T-cell factor-4 variant M; T-cell factor-4 variant X2; T-cell-specific transcription factor 4; TCF-4T-cell factor-4 variant F; TCF4T-cell factor-4 variant G; transcription factor 7-like 2 (T-cell specific, HMG-box); transcription factor 7-like 2 TCF7L2 TCF-4, TCF4 transcription factor 7 like 2 transcription factor 7-like 2|HMG box transcription factor 4|T-cell factor 4|T-cell-specific transcription factor 4|hTCF-4|transcription factor 7-like 2 (T-cell specific, HMG-box)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCF7L2, check out the TCF7L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCF7L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCF7L2 Rabbit Monoclonal Antibody (M00431)
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No publications found for M00431
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TCF7L2 Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-TCF7L2 Rabbit Monoclonal antibody M00431 available?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TCF7L2 Rabbit Monoclonal antibody M00431 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-30
Question
I would like to test anti-TCF7L2 Rabbit Monoclonal antibody M00431 on human liver for research purposes, then I may be interested in using anti-TCF7L2 Rabbit Monoclonal antibody M00431 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
The products we sell, including anti-TCF7L2 Rabbit Monoclonal antibody M00431, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-24
Question
Will anti-TCF7L2 Rabbit Monoclonal antibody M00431 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
According to the expression profile of liver, TCF7L2 is highly expressed in liver. So, it is likely that anti-TCF7L2 Rabbit Monoclonal antibody M00431 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-TCF7L2 Rabbit Monoclonal antibody M00431 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The anti-TCF7L2 Rabbit Monoclonal antibody (M00431) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-17