Data & Images
|Product Name||Anti-TdT Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for DNA nucleotidylexotransferase(DNTT) detection. Tested with WB in Human.|
|Cite This Product||Anti-TdT Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9162)|
|Replacement Item||This antibody may replace the following items: sc-19441|sc-292644|sc-393710|sc-398238|sc-59393 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||DNA nucleotidylexotransferase|
|Molecular Weight||58536 MW|
|Protein Function||Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. .|
|Sequence Similarities||Belongs to the DNA polymerase type-X family.|
|Subcellular Localization||Nucleus .|
|Alternative Names||DNA nucleotidylexotransferase;22.214.171.124 ;Terminal addition enzyme;Terminal deoxynucleotidyltransferase;Terminal transferase ;DNTT;TDT ;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|dna synthesis|dna polymerases||
Background for DNA nucleotidylexotransferase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TdT Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TdT Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti TDT (PB9162) at 0.5ug/ml
WB: Recombinant Human TDT Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti TDT (PB9162) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,