|Sample Size:||30ug for $99, contact us for details|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-Tenascin Antibody (Monoclonal, BC-24)|
|Description||Mouse IgG monoclonal antibody for Tenascin, tenascin N (TNN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tenascin Antibody (Monoclonal, BC-24) BC-24 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1094)|
|Replacement Item||This antibody may replace the following items: sc-54349 from Santa Cruz Biotechnology.|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||173250 MW|
|Protein Function||TNN: Involved in neurite outgrowth and cell migration inhippocampal explants.|
|Sequence Similarities||Belongs to the tenascin family.|
|Subcellular Localization||TNN: Secreted, extracellular space, extracellularmatrix.
Tnn: Secreted, extracellular space, extracellularmatrix.
|Alternative Names||Protein Tnn ;Tnn ;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|troponin| cardiovascular|heart|contractility|contractile proteins||
Background for Tenascin-N
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Tenascin Antibody (Monoclonal, BC-24) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, -
Western blot, 1-2μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Tenascin Antibody (Monoclonal, BC-24) Images
Click the images to enlarge.
IHC(P): Human Mammary Cancer Tissue