Anti-TGF beta Receptor I/TGFBR1 Antibody
Rabbit IgG polyclonal antibody for TGF-beta receptor type-1(TGFBR1) detection. Tested with WB, IHC-P in Human;Rat;Mouse. Cited in 3 publication(s).
Anti-TGF beta Receptor I/TGFBR1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-TGF beta Receptor I/TGFBR1 Antibody
See all TGF-beta RI/ALK-5 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for TGF BETA RECEPTOR I/TGFBR1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. TGF BETA RECEPTOR I/TGFBR1 information: Molecular Weight: 55960 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction; Tissue Specificity: Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.|
|Cite This Product||Anti-TGF beta Receptor I/TGFBR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1731)|
|Similar Products From
|Anti-TGF beta Receptor I/TGFBR1 Antibody may replace the following items: sc 137639|sc 137638|sc 137640.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human TGFBR1 (281-295aa DYHEHGSLFDYLNRY), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For TGFBR1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7046|
|Species Of This Entry||Human|
|Protein Name||TGF-beta receptor type-1|
|Superfamily||protein kinase superfamily|
|Alternative Names||TGF-beta RI/ALK-5|AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 126.96.36.199; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor|
|Post Tranlational Modifications||Phosphorylation; Methylation; Cleavage; Ubiquitination|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. Cell surface. Membrane raft.|
|Gene Location||9q22.33, on Chromasome 9, gene sequence: NC_000009.12 (99104038..99154192)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TGFBR1 (Source: Uniprot.org, NCBI)
|Protein Function||Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non- canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.|
|Tissue Specificity||Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. Expressed in a variety of cancer cell lines (PubMed:25893292).|
|Interacting Proteins||FKBP1A; LRG1; TGFB1; YWHAZ|
|Related Diseases||Malignant Neoplasms; Neoplasms; Marfan Syndrome; Aneurysm; Loeys-dietz Syndrome; Aortic Aneurysm; Aortic Aneurysm, Thoracic; Colorectal Cancer; Carcinoma; Connective Tissue Diseases; Cleft Palate; Colorectal Neoplasms; Malignant Neoplasm Of Breast; Mammary Neoplasms; Orbital Separation Excessive; Pathological Dilatation; Dissecting Aortic Aneurysm; Cell Transformation, Neoplastic; Cleft Uvula; Adenocarcinoma|
|Related Pathways||Pathogenesis; Cell Proliferation; Secretion; Localization; Cell Growth; Methylation; Ovulation; Cell Cycle; Angiogenesis; Cell Migration; Oncogenesis; Dna Methylation; Regeneration; Wound Healing; Bone Resorption; Rna Interference; Cell Adhesion; Developmental Process; Lung Development; Vasculogenesis|
|Background||TGFBR1, Transforming growth factor, beta receptor I is a TGF beta receptor. TGFBR1 is its human gene. The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome(LDAS). TGFB1 regulates cell cycle progression by a unique signaling mechanism that involves its binding to TGFBR2 and activation of TGFBR1. Both are transmembrane serine/threonine receptor kinases. The TGFBR1 receptor may be inactivated in many of the cases of human tumor cells refractory to TGFB-mediated cell cycle arrest. Vellucci and Reiss(1997) reported that the TGFBR1 gene is approximately 31 kb long and contains 9 exons. The organization of the segment of the gene that encodes the C-terminal portion of the serine/threonine kinase domain appears to be highly conserved among members of the gene family.|
|Scientific References||PMID: 8242743 by Franzen P., et al. Cloning of a TGF beta type I receptor that forms a heteromeric complex with the TGF beta type II receptor.|
PMID: 9417915 by Vellucci V.F., et al. Cloning and genomic organization of the human transforming growth factor-beta type I receptor gene.
Our Boster Quality Guarantee for Anti-TGF beta Receptor I/TGFBR1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti TGFBR1 (PA1731) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 56KD
Observed bind size: 56KD
IHC(P): Human Mammary Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 3
|Effects of AT1 receptor antagonist, losartan, on rat hepatic fibrosis induced by CCl4||PubMed ID 11780431|
|Effects of interferon-alpha on expression of hepatic stellate cell and transforming growth factor-?1 and ?-smooth muscle actin in rats with hepatic fibrosis||PubMed ID 21464546|
|Different localization and expression of protein kinase C-beta in kidney cortex of diabetic nephropathy mice and its role in telmisartan treatment||PubMed ID 26279755|
Order Product (PA1731)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.