Anti-Thrombospondin/THBS1 Antibody Picoband™

Thrombospondin-1 antibody

Boster Bio Anti-Thrombospondin/THBS1 Antibody Picoband™ catalog # PB9450. Tested in WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: PB9450
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Thrombospondin/THBS1 Antibody Picoband™

View all Thrombospondin-1 Antibodies

SKU/Catalog Number

PB9450

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Thrombospondin/THBS1 Antibody Picoband™ catalog # PB9450. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Thrombospondin/THBS1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9450)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human THBS1, different from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9450 is reactive to THBS1 in Human

Applications

PB9450 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

190 kDa

Calculated molecular weight

129.383kDa

Background of Thrombospondin-1

THBS1 is also known as Thrombospondin 1, TSP1. The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Also this protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. In addition, the thrombospondin-1 protein is a member of the thrombospondinfamily. It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue. Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1. This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, αv integrins, β1 integrins, syndecan, and integrin-associated protein (IAP or CD47). It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase, thrombin, cathepsin, and elastase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For THBS1 (Source: Uniprot.org, NCBI)

Gene Name

THBS1

Full Name

Thrombospondin-1

Weight

129.383kDa

Superfamily

thrombospondin family

Alternative Names

THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1 THBS1 THBS, THBS-1, TSP, TSP-1, TSP1 thrombospondin 1 thrombospondin-1|glycoprotein G|thrombospondin-1p180|thrombospondin-p50

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on THBS1, check out the THBS1 Infographic

THBS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for THBS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9450 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Reactive oxygen species%u2013mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression

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6 Customer Q&As for Anti-Thrombospondin/THBS1 Antibody Picoband™

Question

I am looking for to test anti-Thrombospondin/THBS1 antibody PB9450 on human liver for research purposes, then I may be interested in using anti-Thrombospondin/THBS1 antibody PB9450 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

The products we sell, including anti-Thrombospondin/THBS1 antibody PB9450, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-03

Question

I see that the anti-Thrombospondin/THBS1 antibody PB9450 works with WB, what is the protocol used to produce the result images on the product page?

M. Roberts

Verified customer

Asked: 2019-07-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-29

Question

Can you help my question with product PB9450, anti-Thrombospondin/THBS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9450 anti-Thrombospondin/THBS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-31

Question

We are currently using anti-Thrombospondin/THBS1 antibody PB9450 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?

S. Lewis

Verified customer

Asked: 2018-09-03

Answer

The anti-Thrombospondin/THBS1 antibody (PB9450) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-03

Question

I was wanting to use your anti-Thrombospondin/THBS1 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?

S. Patel

Verified customer

Asked: 2015-05-15

Answer

You can see on the product datasheet, PB9450 anti-Thrombospondin/THBS1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-05-15

Question

Is a blocking peptide available for product anti-Thrombospondin/THBS1 antibody (PB9450)?

K. Krishna

Verified customer

Asked: 2014-02-27

Answer

We do provide the blocking peptide for product anti-Thrombospondin/THBS1 antibody (PB9450). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-02-27

Order DetailsPrice
PB9450

100μg

$370
PB9450-10ug

10μg sample (liquid)

$99
PB9450-Biotin

100 μg Biotin conjugated

$570
PB9450-Cy3

100 μg Cy3 conjugated

$570
PB9450-Dylight488

100 μg Dylight488 conjugated

$570
PB9450-Dylight550

100 μg Dylight550 conjugated

$570
PB9450-Dylight594

100 μg Dylight594 conjugated

$570
PB9450-FITC

100 μg FITC conjugated

$570
PB9450-HRP

100 μg HRP conjugated

$570
PB9450-APC

100 μg APC conjugated

$670
PB9450-PE

100 μg PE conjugated

$670
PB9450-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9450
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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