|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-KIM1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Hepatitis A virus cellular receptor 1(HAVCR1) detection. Tested with WB, IHC-P, ICC in Human.|
|Cite This Product||Anti-KIM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1624)|
|Replacement Item||This antibody may replace the following items: sc-12374|sc-367179|sc-393122|sc-393146|sc-8891 from Santa Cruz Biotechnology.|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TIM 1(332-348aa IKALQNAVEKEVQAEDN).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Hepatitis A virus cellular receptor 1(HAVcr-1)|
|Molecular Weight||38720 MW|
|Protein Function||May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. .|
|Tissue Specificity||Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. .|
|Sequence Similarities||Belongs to the immunoglobulin superfamily. TIM family.|
|Subcellular Localization||Membrane ; Single-pass type I membrane protein .|
|Alternative Names||Hepatitis A virus cellular receptor 1;HAVcr-1;Kidney injury molecule 1;KIM-1;T-cell immunoglobulin and mucin domain-containing protein 1;TIMD-1;T-cell immunoglobulin mucin receptor 1;TIM;TIM-1;T-cell membrane protein 1;HAVCR1;KIM1, TIM1, TIMD1;|
|Research Areas|||immunology|adaptive immunity|t cells|non-cd| microbiology|interspecies interaction|host virus interaction| immunology|immune system diseases|allergens||
Background for Hepatitis A virus cellular receptor 1(HAVcr-1)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-KIM1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-KIM1 Antibody Images
Click the images to enlarge.
Lane 1: SMMC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: M231 Cell Lysate
Lane 5: M453 Cell Lysate
IHC(P): Human Tonsil Tissue
ICC: K562 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,