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SKU:PA1385
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-TIMP-1 Antibody
SKU/Catalog Number PA1385
Description Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 1(TIMP1) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-TIMP-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1385)
Replacement Item This antibody may replace the following items: sc-1310|sc-21734|sc-365905|sc-5290|sc-5538|sc-56489|sc-58435|sc-6832|sc-6832-R|sc-7956|sc-80995 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP-1(189-207aa CLPREPGLCTWQSLRSQIA).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TIMP1
Protein Name Metalloproteinase inhibitor 1
Molecular Weight 23171 MW
Protein Function Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. .
Tissue Specificity Detected in rheumatoid synovial fluid (at protein level). .
Sequence Similarities Belongs to the protease inhibitor I35 (TIMP) family.
Subcellular Localization Secreted .
Uniprot ID P01033
Alternative Names Metalloproteinase inhibitor 1;Erythroid-potentiating activity;EPA;Fibroblast collagenase inhibitor;Collagenase inhibitor;Tissue inhibitor of metalloproteinases 1;TIMP-1;TIMP1;CLGI, TIMP;
Research Areas |cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|timp| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|timp1 / timp2| cancer|invasion/microenvironment|timps| cell biology|proteolysis / ubiquitin|protease inhibitors|metalloprotease inhibitors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Metalloproteinase inhibitor 1

TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases(MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. TIMP was found to be located about 22 cM proximal to OTC(300461).

Anti-TIMP-1 Antibody Images

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Anti-TIMP-1 Antibody
Anti-TIMP-1 antibody, PA1385, Western blotting
Lane 1: Recombinant Human TIMP-1 Protein 10ng
Lane 2: Recombinant Human TIMP-1 Protein 5ng
Lane 3: Recombinant Human TIMP-1 Protein 2.5ng
Anti-TIMP-1 Antibody
Anti-TIMP-1 antibody, PA1385, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Liu R, Ahmed Km, Nantajit D, Rosenthal Fs, Hai Cx, Li Jj. Int J Mol Med. 2007 Jun;19(6):865-73. Therapeutic Effects Of Alpha-Lipoic Acid On Bleomycin-Induced Pulmonary Fibrosis In Rats.
Song Y, Zou H, Wang G, Yang H, Xie Z, Bi J. Neural Regen Res. 2012 Jun 15;7(17):1325-30. Doi: 10.3969/J.Issn.1673-5374.2012.17.007. Matrix Metalloproteinase-9 And Tissue Inhibitor Of Metalloproteinase-1 Expression In Early Focal Cerebral Infarctio...
Zhao Q, Xue Y, Liu Zd, Li H, Wang Jf, Li Zj, Wang Ym, Dong P, Xue Ch. J Food Sci. 2010 Nov-Dec;75(9):H280-8. Doi: 10.1111/J.1750-3841.2010.01837.X. Epub 2010 Oct 15. Differential Effects Of Sulfated Triterpene Glycosides, Holothurin A1, And 24-Deh...
Hu P, Wang J, Zhao Xq, Hu B, Lu L, Qin Yh. Mol Biol Rep. 2013 Feb;40(2):1429-41. Doi: 10.1007/S11033-012-2186-7. Epub 2012 Oct 17. Overexpressed C-Type Natriuretic Peptide Serves As An Early Compensatory Response To Counteract Extracellular Matrix...
Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...
Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.
Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.
Wu L, Zhang W, Tang Yh, Li H, Chen By, Zhang Gm, Deng Cq. Phytomedicine. 2010 Mar;17(3-4):233-40. Doi: 10.1016/J.Phymed.2009.07.021. Epub 2009 Sep 11. Effect Of Total Saponins Of "Panax Notoginseng Root" On Aortic Intimal Hyperplasia And The Expre...
Jiang Z, Han B, Li H, Yang Y, Liu W. Carbohydr Polym. 2015 Sep 20;129:1-8. Doi: 10.1016/J.Carbpol.2015.04.040. Epub 2015 Apr 27. Carboxymethyl Chitosan Represses Tumor Angiogenesis In Vitro And In Vivo.
Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon ...
Deng Cl, Ling St, Liu Xq, Zhao Yj, Lv Yf. Exp Ther Med. 2015 Mar;9(3):992-998. Epub 2015 Jan 20. Decreased Expression Of Matrix Metalloproteinase-1 In The Maternal Umbilical Serum, Trophoblasts And Decidua Leads To Preeclampsia.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to epo antibody, timp 1 antibody, timp antibody, timp1 antibody, timp-1 antibody