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SKU:PA1078
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-TIMP-4 Antibody
Description Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 4(TIMP4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-TIMP-4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1078)
Replacement Item This antibody may replace the following items: sc-30076|sc-9374|sc-9375 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP4(208-224aa YRGHLPLRKEFVDIVQP), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Liquid
Contents Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TIMP4
Protein Name Metalloproteinase inhibitor 4
Molecular Weight 25503 MW
Protein Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9.
Tissue Specificity Abundant in heart and present at low levels in many other tissues.
Sequence Similarities Belongs to the protease inhibitor I35 (TIMP) family.
Subcellular Localization Secreted.
Uniprot ID Q99727
Alternative Names Metalloproteinase inhibitor 4;Tissue inhibitor of metalloproteinases 4;TIMP-4;TIMP4;
Research Areas |cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|timp| cancer|invasion/microenvironment|ecm enzymes|timps|extracellular matrix| cell biology|proteolysis / ubiquitin|protease inhibitors|metalloprotease inhibitors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Metalloproteinase inhibitor 4

Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. And this gene is mapped to chromosome 3p25. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Anti-TIMP-4 Antibody Images

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Anti-TIMP-4 Antibody
Anti-TIMP4 antibody, PA1078, Western blotting
All lanes: Anti TIMP4 (PA1078) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
Anti-TIMP-4 Antibody
Anti-TIMP4 antibody, PA1078, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-TIMP-4 Antibody
Anti-TIMP4 antibody, PA1078, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.