|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-TJP2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Tight junction protein ZO-2(TJP2) detection. Tested with WB, IHC-P, IHC-F in Human.|
|Cite This Product||Anti-TJP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1957)|
|Replacement Item||This antibody may replace the following items: sc-50103|sc-50105|sc-50101 from Santa Cruz Biotechnology.|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TJP2(1171-1190aa SEHSKRGYYGQSARYRDTEL).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tight junction protein ZO-2|
|Molecular Weight||133958 MW|
|Protein Function||Plays a role in tight junctions and adherens junctions.|
|Tissue Specificity||This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues. .|
|Sequence Similarities||Belongs to the MAGUK family.|
|Subcellular Localization||Cell junction, adherens junction. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Nucleus . Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures. .|
|Alternative Names||Tight junction protein ZO-2;Tight junction protein 2;Zona occludens protein 2;Zonula occludens protein 2;TJP2;X104, ZO2;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cell adhesion|tight junctions||
Background for Tight junction protein ZO-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TJP2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TJP2 Antibody Images
Click the images to enlarge.
Lane 1: 293T Cell Lysate
Lane 2: MCF-7 Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,