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SKU:PA1484
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-TLR4 Antibody
SKU/Catalog Number PA1484
Description Rabbit IgG polyclonal antibody for Toll-like receptor 4(TLR4) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-TLR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1484)
Replacement Item This antibody may replace the following items: sc-30002|sc-13593|sc-10741|sc-293072|sc-12511|sc-16240|sc-8694|sc-52962|sc-13591|sc-8694-R from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4(50-68aa DNLPFSTKNLDLSFNPLRH).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TLR4
Protein Name Toll-like receptor 4
Molecular Weight 95680 MW
Protein Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species- specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. .
Tissue Specificity Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Sequence Similarities Belongs to the Toll-like receptor family.
Subcellular Localization Cell membrane; Single-pass type I membrane protein. Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis. .
Uniprot ID O00206
Alternative Names Toll-like receptor 4;hToll;CD284;TLR4;
Research Areas |immunology|innate immunity|macrophage / inflamm.| immunology|immunoglobulins|receptors| microbiology|interspecies interaction|host virus interaction| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators|adaptive immunity|regulatory t cells|tlr signaling|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Toll-like receptor 4

TLR4, Toll-like receptor 4, is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. TLR4, the human homolog of Drosophila Toll, is a type I transmembrane protein with an extracellular domain consisting of a leucine-rich repeat region and an intracellular domain homologous to that of human interleukin-1 receptor. The TLR4 gene is mapped to chromosome 9q32-q33 by fluorescence in situ hybridization.It detects lipopolysaccharide from Gram-negative bacteria and is thus important in the activation of the innate immune system.The protein encoded by this gene is a member of the Toll-like receptor(TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns(PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

Anti-TLR4 Antibody Images

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Anti-TLR4 Antibody
Anti-TLR4 antibody, PA1484, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Anti-TLR4 Antibody
Anti-TLR4 antibody, PA1484, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Publications

Hong-Xia Zhou1, Ling-Huan Gao1, , , Ling-Li Meng2, Yu-Xin Zhang1, Zi-Feng Wei1, Dao-Wen Si1 Asian Pacific Journal of Tropical Medicine Available online 19 January 2017 Preventive and therapeutic effect of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage
Tu Ct, Yao Qy, Xu Bl, Wang Jy, Zhou Ch, Zhang Sc. Food Chem Toxicol. 2012 Sep;50(9):3343-51. Doi: 10.1016/J.Fct.2012.05.050. Epub 2012 Jun 7. Protective Effects Of Curcumin Against Hepatic Fibrosis Induced By Carbon Tetrachloride: Modulation Of Hi...
Xu N, Yu Zh, Yao Qs, Wang Zq, Qu Hl, Sun Y, Liu J, Jin Cz. J Immunoassay Immunochem. 2013;34(3):219-31. Doi: 10.1080/15321819.2012.699490. Ppar-?? And Tollip Are Associated With Toll-Like Receptors In Colitis Rats.
Fu Hy, Li C, Yang W, Gai Xd, Jia T, Lei Ym, Li Y. Acta Histochem. 2013 Mar;115(2):151-7. Doi: 10.1016/J.Acthis.2012.06.002. Epub 2012 Jun 29. Foxp3 And Tlr4 Protein Expression Are Correlated In Non-Small Cell Lung Cancer: Implications For Tumor Pr...
Wang Yz, Tian Ff, Liu H, Zhang W, Li J, Xiao B, Zhou Wb. J Neuroimmunol. 2013 Apr 15;257(1-2):67-75. Doi: 10.1016/J.Jneuroim.2013.01.006. Epub 2013 Feb 12. Macrophage Migration Inhibitory Factor Is Necessary For The Lipo-Oligosaccharide-Induced Re...
Yang Ss, Tang L, Li Rg, Ge Gh, Qu Xk, Ma Jw, Qiao Zy, Zhang L, Liu Hj, Hou Ym, Cao H, Hao Zm, Cheng Wb, Wang Hw. Neuro Endocrinol Lett. 2014;35(1):80-6. The Effects Of Subclinical Hypothyroidism On Serum Lipid Level And Tlr4 Expression Of Monocyte...
Yang Ss, Li R, Qu X, Fang W, Quan Z. Cell Immunol. 2012 Sep;279(1):96-102. Doi: 10.1016/J.Cellimm.2012.09.008. Epub 2012 Oct 1. Atorvastatin Decreases Toll-Like Receptor 4 Expression And Downstream Signaling In Human Monocytic Leukemia Cells.
Liu X, Hu R, Lian H, Liu Y, Liu J, Liu J, Lin G, Liu L, Duan X, Yong Kt, Ye L. Int J Nanomedicine. 2015 May 20;10:3651-62. Doi: 10.2147/Ijn.S81395. Ecollection 2015. Dual-Color Immunofluorescent Labeling With Quantum Dots Of The Diabetes-Associate...
Xu M, Wang Kn, Wu K, Wang Xp. Gut Liver. 2015 May 23;9(3):411-6. Doi: 10.5009/Gnl14050. Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ??b And Toll-Like Receptor 4 Expression In Rats With Acute Necrotizing Pancreatitis.
Ou Jm, Zhang Xp, Wu Cj, Wu Dj, Yan P. J Zhejiang Univ Sci B. 2012 Nov;13(11):919-31. Doi: 10.1631/Jzus.B1100351. Effects Of Dexamethasone And Salvia Miltiorrhiza On Multiple Organs In Rats With Severe Acute Pancreatitis.
Cui L, Feng L, Zhang Zh, Jia Xb. Int Immunopharmacol. 2014 Nov;23(1):294-303. Doi: 10.1016/J.Intimp.2014.09.005. Epub 2014 Sep 18. The Anti-Inflammation Effect Of Baicalin On Experimental Colitis Through Inhibiting Tlr4/Nf-??b Pathway Activation.
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
Yuan Z, Zhang J, Yang C. Evid Based Complement Alternat Med. 2014;2014:620359. Doi: 10.1155/2014/620359. Epub 2014 Aug 17. Ligusticum Wallichii Extract Inhibited The Expression Of Il-1?? After Ami In Rats.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tlr4 antibody, tlr 4 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody