Anti-TLR7 Antibody Picoband™

TLR7 antibody

Boster Bio Anti-TLR7 Antibody Picoband™ catalog # PB9452. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9452
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, ICC, WB

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Product Name

Anti-TLR7 Antibody Picoband™

View all TLR7 Antibodies

SKU/Catalog Number

PB9452

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TLR7 Antibody Picoband™ catalog # PB9452. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TLR7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9452)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9452 is reactive to TLR7 in Human, Mouse, Rat

Applications

PB9452 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

121 kDa

Calculated molecular weight

120.922kDa

Background of TLR7

TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunocytochemistry , 0.5-1μg/ml, Human, -
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For TLR7 (Source: Uniprot.org, NCBI)

Gene Name

TLR7

Full Name

Toll-like receptor 7

Weight

120.922kDa

Superfamily

Toll-like receptor family

Alternative Names

TLR7; toll-like receptor 7 TLR7 IMD74-like, TLR7 toll like receptor 7 toll-like receptor 7|toll-like receptor 7-like

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TLR7, check out the TLR7 Infographic

TLR7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TLR7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9452 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Esancy K, Condon L, Feng J, Kimball C, Curtright A, Dhaka A. Elife. 2018 Mar 21;7. pii: e32036. doi: 10.7554/eLife.32036. A zebrafish and mouse model for selective pruritus via direct activation of TRPA1

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16 Customer Q&As for Anti-TLR7 Antibody Picoband™

Question

I am looking for using your anti-TLR7 antibody for i-kappab phosphorylation studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

We appreciate your inquiry. This PB9452 anti-TLR7 antibody is validated on colo320 whole cell lysate, jurkat whole cell lysate, mouse thymus tissue, rat thymus tissue, human placenta tissue. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-20

Question

I was wanting to use your anti-TLR7 antibody for ICC for rat placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat placenta identification?

L. Carter

Verified customer

Asked: 2020-03-20

Answer

You can see on the product datasheet, PB9452 anti-TLR7 antibody has been validated for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-20

Question

We are currently using anti-TLR7 antibody PB9452 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

The anti-TLR7 antibody (PB9452) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-28

Question

Do you have a BSA free version of anti-TLR7 antibody PB9452 available?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TLR7 antibody PB9452 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-19

Question

We were well pleased with the WB result of your anti-TLR7 antibody. However we have been able to see positive staining in leukocyte endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is TLR7 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

From literature, leukocyte does express TLR7. Generally TLR7 expresses in endoplasmic reticulum membrane. Regarding which tissues have TLR7 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 11022119, 11022120, 19924287
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-22

Question

Will PB9452 anti-TLR7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

As indicated on the product datasheet, PB9452 anti-TLR7 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-13

Question

We purchased anti-TLR7 antibody for IHC on placenta a few months ago. I am using human, and We are going to use the antibody for Flow Cytometry next. I would like examining placenta as well as testis in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

I have checked the website and datasheets of our anti-TLR7 antibody and it appears that PB9452 has been tested on human in both IHC and Flow Cytometry. Thus PB9452 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-27

Question

Can you help my question with product PB9452, anti-TLR7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9452 anti-TLR7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-07

Question

We have seen staining in rat leukocyte. Are there any suggestions? Is anti-TLR7 antibody supposed to stain leukocyte positively?

C. Roberts

Verified customer

Asked: 2019-02-28

Answer

From what I have seen in literature leukocyte does express TLR7. From what I have seen in Uniprot.org, TLR7 is expressed in leukocyte, placenta, testis, among other tissues. Regarding which tissues have TLR7 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 11022119, 11022120, 19924287
Testis, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-02-28

Question

Is this PB9452 anti-TLR7 antibody reactive to the isotypes of TLR7?

Verified Customer

Verified customer

Asked: 2019-02-13

Answer

The immunogen of PB9452 anti-TLR7 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7 (1020-1047aa QAHPYFWQCLKNALATDNHVAYSQVFKE), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-13

Question

Is a blocking peptide available for product anti-TLR7 antibody (PB9452)?

Verified Customer

Verified customer

Asked: 2019-01-01

Answer

We do provide the blocking peptide for product anti-TLR7 antibody (PB9452). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-01

Question

See attached the WB image, lot number and protocol we used for placenta using anti-TLR7 antibody PB9452. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-09-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-20

Question

My question regards to test anti-TLR7 antibody PB9452 on rat placenta for research purposes, then I may be interested in using anti-TLR7 antibody PB9452 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

The products we sell, including anti-TLR7 antibody PB9452, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-11

Question

I see that the anti-TLR7 antibody PB9452 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-05-29

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-05-29

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-TLR7 antibody PB9452. Let me know if you need anything else.

S. Carter

Verified customer

Asked: 2016-12-26

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-12-26

Question

Does anti-TLR7 antibody PB9452 work for ICC with placenta?

R. Dhar

Verified customer

Asked: 2013-10-02

Answer

According to the expression profile of placenta, TLR7 is highly expressed in placenta. So, it is likely that anti-TLR7 antibody PB9452 will work for ICC with placenta.

Boster Scientific Support

Answered: 2013-10-02

Order DetailsPrice
PB9452

100μg

$370
PB9452-10ug

10μg sample (liquid)

$99
PB9452-Biotin

100 μg Biotin conjugated

$570
PB9452-Cy3

100 μg Cy3 conjugated

$570
PB9452-Dylight488

100 μg Dylight488 conjugated

$570
PB9452-Dylight550

100 μg Dylight550 conjugated

$570
PB9452-Dylight594

100 μg Dylight594 conjugated

$570
PB9452-FITC

100 μg FITC conjugated

$570
PB9452-HRP

100 μg HRP conjugated

$570
PB9452-APC

100 μg APC conjugated

$670
PB9452-PE

100 μg PE conjugated

$670
PB9452-carrier-free

Carrier Free

$370

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