Product Info Summary
SKU: | PB9452 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-TLR7 Antibody Picoband™
SKU/Catalog Number
PB9452
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TLR7 Antibody Picoband™ catalog # PB9452. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9452)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9452 is reactive to TLR7 in Human, Mouse, Rat
Applications
PB9452 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
121 kDa
Calculated molecular weight
120.922kDa
Background of TLR7
TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunocytochemistry , 0.5-1μg/ml, Human, -
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 Whole Cell Lysate
Lane 2: COLO320 Whole Cell Lysate
Lane 3: JURKAT Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.
Click image to see more details
Figure 2. IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).
TLR7 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).
TLR7 was detected in paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).
TLR7 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).
TLR7 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).
TLR7 was detected in immunocytochemical section of a549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. Flow Cytometry analysis of H-PBMC cells using anti-TLR7 antibody (PB9452).
Overlay histogram showing H-PBMC cells stained with PB9452 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR7 Antibody (PB9452,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TLR7 (Source: Uniprot.org, NCBI)
Gene Name
TLR7
Full Name
Toll-like receptor 7
Weight
120.922kDa
Superfamily
Toll-like receptor family
Alternative Names
TLR7; toll-like receptor 7 TLR7 IMD74-like, TLR7 toll like receptor 7 toll-like receptor 7|toll-like receptor 7-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR7, check out the TLR7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TLR7 Antibody Picoband™ (PB9452)
Hello CJ!
PB9452 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Esancy K, Condon L, Feng J, Kimball C, Curtright A, Dhaka A. Elife. 2018 Mar 21;7. pii: e32036. doi: 10.7554/eLife.32036. A zebrafish and mouse model for selective pruritus via direct activation of TRPA1
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-TLR7 Antibody Picoband™
Question
I am looking for using your anti-TLR7 antibody for i-kappab phosphorylation studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
We appreciate your inquiry. This PB9452 anti-TLR7 antibody is validated on colo320 whole cell lysate, jurkat whole cell lysate, mouse thymus tissue, rat thymus tissue, human placenta tissue. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-20
Question
I was wanting to use your anti-TLR7 antibody for ICC for rat placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat placenta identification?
L. Carter
Verified customer
Asked: 2020-03-20
Answer
You can see on the product datasheet, PB9452 anti-TLR7 antibody has been validated for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-20
Question
We are currently using anti-TLR7 antibody PB9452 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The anti-TLR7 antibody (PB9452) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
Do you have a BSA free version of anti-TLR7 antibody PB9452 available?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TLR7 antibody PB9452 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-19
Question
We were well pleased with the WB result of your anti-TLR7 antibody. However we have been able to see positive staining in leukocyte endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is TLR7 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
From literature, leukocyte does express TLR7. Generally TLR7 expresses in endoplasmic reticulum membrane. Regarding which tissues have TLR7 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 11022119, 11022120, 19924287
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-22
Question
Will PB9452 anti-TLR7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
As indicated on the product datasheet, PB9452 anti-TLR7 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-13
Question
We purchased anti-TLR7 antibody for IHC on placenta a few months ago. I am using human, and We are going to use the antibody for Flow Cytometry next. I would like examining placenta as well as testis in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
I have checked the website and datasheets of our anti-TLR7 antibody and it appears that PB9452 has been tested on human in both IHC and Flow Cytometry. Thus PB9452 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-27
Question
Can you help my question with product PB9452, anti-TLR7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9452 anti-TLR7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-07
Question
We have seen staining in rat leukocyte. Are there any suggestions? Is anti-TLR7 antibody supposed to stain leukocyte positively?
C. Roberts
Verified customer
Asked: 2019-02-28
Answer
From what I have seen in literature leukocyte does express TLR7. From what I have seen in Uniprot.org, TLR7 is expressed in leukocyte, placenta, testis, among other tissues. Regarding which tissues have TLR7 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 11022119, 11022120, 19924287
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-02-28
Question
Is this PB9452 anti-TLR7 antibody reactive to the isotypes of TLR7?
Verified Customer
Verified customer
Asked: 2019-02-13
Answer
The immunogen of PB9452 anti-TLR7 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7 (1020-1047aa QAHPYFWQCLKNALATDNHVAYSQVFKE), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-13
Question
Is a blocking peptide available for product anti-TLR7 antibody (PB9452)?
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
We do provide the blocking peptide for product anti-TLR7 antibody (PB9452). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-01
Question
See attached the WB image, lot number and protocol we used for placenta using anti-TLR7 antibody PB9452. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-20
Question
My question regards to test anti-TLR7 antibody PB9452 on rat placenta for research purposes, then I may be interested in using anti-TLR7 antibody PB9452 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
The products we sell, including anti-TLR7 antibody PB9452, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-11
Question
I see that the anti-TLR7 antibody PB9452 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-29
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-29
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-TLR7 antibody PB9452. Let me know if you need anything else.
S. Carter
Verified customer
Asked: 2016-12-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-26
Question
Does anti-TLR7 antibody PB9452 work for ICC with placenta?
R. Dhar
Verified customer
Asked: 2013-10-02
Answer
According to the expression profile of placenta, TLR7 is highly expressed in placenta. So, it is likely that anti-TLR7 antibody PB9452 will work for ICC with placenta.
Boster Scientific Support
Answered: 2013-10-02