Product Info Summary
SKU: | A30547 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-TMEPA PMEPA1 Antibody
SKU/Catalog Number
A30547
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TMEPA PMEPA1 Antibody (Catalog# A30547). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMEPA PMEPA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30547)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TMEPA. Immunogen sequence location: 101-150
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30547 is reactive to PMEPA1 in Human, Mouse, Rat
Applications
A30547 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
31.609kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HT-29 cells, using TMEPA Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human prostate tissue, using TMEPA Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HUVEC cells, using TMEPA Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PMEPA1 (Source: Uniprot.org, NCBI)
Gene Name
PMEPA1
Full Name
Protein TMEPAI
Weight
31.609kDa
Superfamily
PMEPA1 family
Alternative Names
prostate transmembrane protein, androgen induced 1; Solid tumor-associated 1 protein; STAG1prostate androgen induced RNA; transmembrane prostate androgen-induced protein PMEPA1 STAG1, TMEPAI prostate transmembrane protein, androgen induced 1 protein TMEPAI|solid tumor-associated 1 protein|transmembrane, prostate androgen induced RNA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PMEPA1, check out the PMEPA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PMEPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEPA PMEPA1 Antibody (A30547)
Hello CJ!
No publications found for A30547
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TMEPA PMEPA1 Antibody
Question
Does A30547 anti-TMEPA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
As indicated on the product datasheet, A30547 anti-TMEPA antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-05
Question
We are currently using anti-TMEPA antibody A30547 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The anti-TMEPA antibody (A30547) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-28
Question
Is there a BSA free version of anti-TMEPA antibody A30547 available?
Verified Customer
Verified customer
Asked: 2017-06-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TMEPA antibody A30547 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-14
Question
My question regarding product A30547, anti-TMEPA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Miller
Verified customer
Asked: 2014-02-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30547 anti-TMEPA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-14