SKU A00002-3
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Applications ELISA, WB

Overview

Product Name Anti-TNF alpha Picoband™ Antibody
SKU/Catalog Number A00002-3
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for TNF alpha detection. Tested with WB, ELISA(Cap) in Human.
Cite This Product Anti-TNF alpha Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002-3)
Host Rabbit
Contents/Buffer Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen E. coli-derived human TNF alpha recombinant protein (Position: V77-L233).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Western blot, 0.1-0.5μg/ml
ELISA(Cap), 1-5μg/ml

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/A/0/A00002 3 TNF alpha primary antibodies WB testing 1.jpg

Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human TNF alpha protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01375
Gene Name TNF
Protein Name tumor necrosis factor
Alternative Names Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; TNF; TNFA; TNFSF2
Subcellular Localization Cell membrane.

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).
Research Areas Immunology, Innate Immunity, Metabolism, Signal Transduction, Tnf Superfamily, Vascular Inflammation

*You can search these to find other products in these research areas.
Background TNF(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

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Order Product (A00002-3)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
1 Unit $240

USD $240

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Troubleshooting

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Publications

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Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model
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CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment
Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication
Effect of Puerarin on Expression of ICAM-1 and TNF-? in Kidneys of Diabetic Rats
Hydrogen sulfide accelerates wound healing in diabetic rats
Molecular responses of radiation-induced liver damage in rats
Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation
Comparison of Serum Levels of Vitamin D and Inflammatory Markers Between Women With Gestational Diabetes Mellitus and Healthy Pregnant Control
Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A
Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury
In Vivo Evaluation of the Ameliorating Effects of Small-Volume Resuscitation with Four Different Fluids on Endotoxemia-Induced Kidney Injury
Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus
Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke
Gu R, Ding X, Tang W, Lei B, Jiang C, Xu G. Front Pharmacol. 2018 Apr 6;9:331. doi: 10.3389/fphar.2018.00331. eCollection 2018. A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal M
Liu Hp, Gao Zh, Cui Sx, Sun Df, Wang Y, Zhao Cr, Lou Hx, Qu Xj. Plos One. 2012;7(3):E33243. Doi: 10.1371/Journal.Pone.0033243. Epub 2012 Mar 14. Inhibition Of Intestinal Adenoma Formation In Apc(Min/+) Mice By Riccardin D, A Natural Product Derive...
Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.
Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.
Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
Zhang Z, Wu Y, Zhao Y, Xiao X, Liu J, Zhou X. Exp Ther Med. 2013 May;5(5):1523-1527. Epub 2013 Mar 22. Dynamic Changes In Hmgb1 Levels Correlate With Inflammatory Responses During Cardiopulmonary Bypass.
Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.