Data & Images
|Product Name||Anti-TNF Alpha Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB in Rat.|
|Cite This Product||Anti-TNF Alpha Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9010)|
|Replacement Item||This antibody may replace the following items: sc-33679|sc-56492|sc-48105|sc-134525|sc-376161|sc-393037|sc-390945 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tumor necrosis factor|
|Molecular Weight||25806 MW|
|Protein Function||Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.|
|Subcellular Localization||Cell membrane ; Single-pass type II membrane protein .|
|Alternative Names||Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2;|
|Research Areas|||immunology|innate immunity|cytokines|tnf superfamily| signal transduction|growth factors/hormones|tnf| cancer|growth factors| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators| metabolism|types of disease|metabolic disorders||
Background for Tumor necrosis factor
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TNF Alpha Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TNF Alpha Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti-TNF alpha(PB9010) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Kidney Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,