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SKU:PB9010
Clonality:Polyclonal
Application:WB
Price: $240.00
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Overview

Product Name Anti-TNF Alpha Picoband™ Antibody
SKU/Catalog Number PB9010
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB in Rat.
Cite This Product Anti-TNF Alpha Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9010)
Replacement Item This antibody may replace the following items: sc-33679|sc-56492|sc-48105|sc-134525|sc-376161|sc-393037|sc-390945 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Tnf
Protein Name Tumor necrosis factor
Molecular Weight 25806 MW
Protein Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Subcellular Localization Cell membrane ; Single-pass type II membrane protein .
Uniprot ID P16599
Alternative Names Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2;
Research Areas |immunology|innate immunity|cytokines|tnf superfamily| signal transduction|growth factors/hormones|tnf| cancer|growth factors| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators| metabolism|types of disease|metabolic disorders|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tumor necrosis factor

TNF alpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Anti-TNF Alpha Picoband™ Antibody Images

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Anti-TNF Alpha Picoband™ Antibody
Anti-TNF alpha Picoband antibody, PB9010-1.jpg
All lanes: Anti-TNF alpha(PB9010) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Kidney Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
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Publications

Gu, R., Tang, W., Lei, B., Ding, X., Jiang, C., & Xu, G. (2017). Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats. Investigative Ophthalmology & Visual Science, 58(9), 3656-3668. doi: 10.1167/iovs.17-22116
Lu Nn, Liu Q, Gu Lg, Ge Sj, Wu J, Ze-Ji Q, Qiu Zj, Zhang Hc, Chao Ex, Yu Zn. Evid Based Complement Alternat Med. 2014;2014:976364. Doi: 10.1155/2014/976364. Epub 2014 Jan 8. Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Ac...
Wu W, Su M, Li T, Wu K, Wu X, Tang Z. Int Immunopharmacol. 2015 Sep;28(1):182-7. Doi: 10.1016/J.Intimp.2015.06.003. Epub 2015 Jun 10. Cantharidin-Induced Liver Injuries In Mice And The Protective Effect Of Vitamin C Supplementation.
Qiang G, Zhang L, Yang X, Xuan Q, Shi L, Zhang H, Chen B, Li X, Zu M, Zhou D, Guo J, Yang H, Du G. Eur J Pharmacol. 2012 Jun 15;685(1-3):156-64. Doi: 10.1016/J.Ejphar.2012.04.028. Epub 2012 Apr 21. Effect Of Valsartan On The Pathological Progressi...
Sun J, Chen Lj, Zhang Gb, Jiang Jt, Zhu M, Tan Y, Wang Ht, Lu Bf, Zhang Xg. Cancer Immunol Immunother. 2010 Aug;59(8):1163-71. Doi: 10.1007/S00262-010-0841-1. Epub 2010 Mar 24. Clinical Significance And Regulation Of The Costimulatory Molecule B7-...
Xie Jb, Zhang X, Li Qh, Xu Zj. Neural Regen Res. 2015 Feb;10(2):219-24. Doi: 10.4103/1673-5374.152374. Inhibition Of Inflammatory Cytokines After Early Decompression May Mediate Recovery Of Neurological Function In Rats With Spinal Cord Injury.
Xia W, Li Dw, Xiang L, Chang Jj, Xia Zl, Han Ej. Chin J Physiol. 2015 Apr 30;58(2):104-13. Doi: 10.4077/Cjp.2015.Bad273. Neuroprotective Effects Of An Aqueous Extract Of Futokadsura Stem In An A??-Induced Alzheimer'S Disease-Like Rat Model.
Li H, Qiu P, Wang J, Niu C, Pan S. Food Funct. 2015 Feb;6(2):470-8. Doi: 10.1039/C4Fo00739E. Effects Of Compound Ginkgo Biloba On Intestinal Permeability In Rats With Alcohol-Induced Liver Injury.
Li C, Chen X, Zhang N, Song Y, Mu Y. Neural Regen Res. 2012 Feb 15;7(5):325-31. Doi: 10.3969/J.Issn.1673-5374.2012.05.001. Gastrodin Inhibits Neuroinflammation In Rotenone-Induced Parkinson'S Disease Model Rats.
Jiang Gt, Chen X, Li D, An Hx, Jiao Jd. Mol Med Rep. 2014 Sep;10(3):1501-8. Doi: 10.3892/Mmr.2014.2323. Epub 2014 Jun 13. Ulinastatin Attenuates Renal Interstitial Inflammation And Inhibits Fibrosis Progression In Rats Under Unilateral Ureteral Ob...
Li G, Ren J, Wang G, Gu G, Hu D, Ren H, Hong Z, Wu X, Liu S, Li J. Int Immunopharmacol. 2014 Feb;18(2):244-8. Doi: 10.1016/J.Intimp.2013.12.014. Epub 2013 Dec 22. T2 Enhances In Situ Level Of Foxp3+ Regulatory Cells And Modulates Inflammatory Cyto...
Jin Hb, Yang Yb, Song Yl, Zhang Yc, Li Yr. Mol Biol Rep. 2012 Dec;39(12):11005-9. Doi: 10.1007/S11033-012-2002-4. Epub 2012 Oct 8. Protective Roles Of Quercetin In Acute Myocardial Ischemia And Reperfusion Injury In Rats.
Ye Q, Zheng Y, Fan S, Qin Z, Li N, Tang A, Ai F, Zhang X, Bian Y, Dang W, Huang J, Zhou M, Zhou Y, Xiong W, Yan Q, Ma J, Li G. Plos One. 2014 Jul 24;9(7):E103298. Doi: 10.1371/Journal.Pone.0103298. Ecollection 2014. Lactoferrin Deficiency Promotes...
Zhao H, Li X, Li N, Liu T, Liu J, Li Z, Xiao H, Li J. Br J Nutr. 2014 Mar 14;111(5):836-46. Doi: 10.1017/S0007114513003115. Epub 2013 Sep 30. Long-Term Resveratrol Treatment Prevents Ovariectomy-Induced Osteopenia In Rats Without Hyperplastic Effe...
Li J, Zhang J, Fu Y, Sun X, Gong T, Jiang J, Zhang Z. J Control Release. 2015 Aug 28;212:19-29. Doi: 10.1016/J.Jconrel.2015.06.011. Epub 2015 Jun 11. Dual Pancreas- And Lung-Targeting Therapy For Local And Systemic Complications Of Acute Pancreati...
Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.
Huang Q, Du J, Fan J, Lv Z, Qian X, Zhang X, Han J, Chen C, Wu F, Jin Y. Med Oncol. 2014 Sep;31(9):144. Doi: 10.1007/S12032-014-0144-Z. Epub 2014 Aug 12. The Effect Of Proinflammatory Cytokines On Il-17Ra Expression In Nsclc.
Guo J, Li F, Wu Q, Gong Q, Lu Y, Shi J. Phytomedicine. 2010 Oct;17(12):950-5. Doi: 10.1016/J.Phymed.2010.03.007. Epub 2010 Apr 9. Protective Effects Of Icariin On Brain Dysfunction Induced By Lipopolysaccharide In Rats.
Cen J, Liu L, Li Ms, He L, Wang Lj, Liu Yq, Liu M, Ji Bs. J Pharm Pharmacol. 2013 May;65(5):665-72. Doi: 10.1111/Jphp.12033. Epub 2013 Feb 26. Alteration In P-Glycoprotein At The Blood-Brain Barrier In The Early Period Of Mcao In Rats.
Cui Wy, Tian Ay, Bai T. Clin Exp Pharmacol Physiol. 2011 Nov;38(11):747-54. Doi: 10.1111/J.1440-1681.2011.05584.X. Protective Effects Of Propofol On Endotoxemia-Induced Acute Kidney Injury In Rats.
Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.
Li Q, Xia Yy, Tang Jc, Wang Ry, Bei Cy, Zeng Y. Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. Doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23. In Vitro And In Vivo Biocompatibility Investigation Of Diamond-Like Carbon Co...
Liu T, Zeng Z, Liu Y, Wang J, Maitz Mf, Wang Y, Liu S, Chen J, Huang N. Acs Appl Mater Interfaces. 2014 Jun 11;6(11):8729-43. Doi: 10.1021/Am5015309. Epub 2014 Apr 23. Surface Modification With Dopamine And Heparin/Poly-L-Lysine Nanoparticles Prov...
Jiang Zy, Guo Yy, Ren Hb, Zou Yf, Fan Ms, Lv Y, Han P, De W, Sun Lz. Placenta. 2012 Jan;33(1):1-7. Doi: 10.1016/J.Placenta.2011.09.004. Epub 2011 Nov 1. Tumor Necrosis Factor (Tnf)-?? Upregulates Progesterone Receptor-A By Activating The Nf-??b Sign...
Zhang Y, Zhang Y, Xie Y, Gao Y, Ma J, Yuan J, Li J, Wang J, Li L, Zhang J, Chu L. J Ethnopharmacol. 2013 Jul 9;148(2):671-81. Doi: 10.1016/J.Jep.2013.05.028. Epub 2013 May 22. Multitargeted Inhibition Of Hepatic Fibrosis In Chronic Iron-Overloaded...
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Int J Mol Sci. 2012;13(7):8379-87. Doi: 10.3390/Ijms13078379. Epub 2012 Jul 5. Astragalus Membranaceus Inhibits Inflammation Via Phospho-P38 Mitogen-Activated Protein Kinase (Mapk) And Nuclear Factor (Nf)-...
Zhang S, Liu X, Goldstein S, Li Y, Ge J, He B, Fei X, Wang Z, Ruiz G. Mol Med Rep. 2013 Jan;7(1):93-8. Doi: 10.3892/Mmr.2012.1159. Epub 2012 Oct 29. Role Of The Jak/Stat Signaling Pathway In The Pathogenesis Of Acute Myocardial Infarction In Rats ...
Jiang Z, Han B, Li H, Yang Y, Liu W. Carbohydr Polym. 2015 Sep 20;129:1-8. Doi: 10.1016/J.Carbpol.2015.04.040. Epub 2015 Apr 27. Carboxymethyl Chitosan Represses Tumor Angiogenesis In Vitro And In Vivo.
Hou Sx, Zhu Wj, Pang Mq, Jeffry J, Zhou Ll. Food Chem Toxicol. 2014 Feb;64:57-64. Doi: 10.1016/J.Fct.2013.11.022. Epub 2013 Nov 26. Protective Effect Of Iridoid Glycosides From Paederia Scandens (Lour.) Merrill (Rubiaceae) On Uric Acid Nephropathy...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Beari...
Chen Yf, Zhao Zq, Wu Zm, Zou Zy, Luo Xj, Li J, Xie C, Liang Y. Int J Clin Exp Pathol. 2014 Dec 1;7(12):8411-20. Ecollection 2014. The Role Of Rip1 And Rip3 In The Development Of Aplastic Anemia Induced By Cyclophosphamide And Busulphan In Mice.
Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.