Anti-TNF alpha Antibody Picoband™

TNF-alpha antibody

Boster Bio Anti-TNF alpha/Tnf Antibody Picoband™ catalog # A00002-5. Tested in ELISA, Flow Cytometry, IF, ICC, and WB applications. This antibody reacts with Mouse and Rat. Cited in 10 publication(s).

Product Info Summary

SKU: A00002-5
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, ICC, WB

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Product Name

Anti-TNF alpha Antibody Picoband™

View all TNF-alpha Antibodies

SKU/Catalog Number

A00002-5

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TNF alpha/Tnf Antibody Picoband™ catalog # A00002-5. Tested in ELISA, Flow Cytometry, IF, ICC, and WB applications. This antibody reacts with Mouse, Rat, offering an essential tool for scientific applications such as cell lysate analysis and breast adenocarcinoma cell line research.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNF alpha Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002-5)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Clone Number

IC-16

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse TNF alpha/Tnf recombinant protein (Position: D89-E185).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00002-5 is reactive to TNF in Mouse, Rat

Applications

A00002-5 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

25.644kDa

Background of TNF-alpha

TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Flow Cytometry, 1-3μg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For TNF (Source: Uniprot.org, NCBI)

Gene Name

TNF

Full Name

Tumor necrosis factor

Weight

25.644kDa

Superfamily

Tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TNF, check out the TNF Infographic

TNF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00002-5 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Hydrogen sulfide accelerates wound healing in diabetic rats

Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response

Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats

Effects of triptolide from Radix Tripterygium wilfordii ( Leigongteng ) on cartilage cytokines and transcription factor NF-κB: a study on induced arthritis in rats

Crocetin attenuates palmitate‐induced insulin insensitivity and disordered tumor necrosis factor‐α and adiponectin expression in rat adipocytes

Relationship between expression of COX-2, TNF-α, IL-6 and autoimmune-type recurrent miscarriage

Effect of Huwentoxin-I on the Fas and TNF apoptosis pathway in the hippocampus of rat with global cerebral ischemia

Gypsophila elegans isoorientin-2″-O-α-l-arabinopyranosyl ameliorates porcine serum-induced immune liver fibrosis by inhibiting NF-κB signaling pathway and suppressing HSC activation

Puerarin exerts the protective effect against chemical induced dysmetabolism in rats

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Order DetailsPrice
A00002-5

100μg

$370
A00002-5-10ug

10μg sample (liquid)

$99
A00002-5-Biotin

100 μg Biotin conjugated

$570
A00002-5-Cy3

100 μg Cy3 conjugated

$570
A00002-5-Dylight488

100 μg Dylight488 conjugated

$570
A00002-5-Dylight550

100 μg Dylight550 conjugated

$570
A00002-5-Dylight594

100 μg Dylight594 conjugated

$570
A00002-5-FITC

100 μg FITC conjugated

$570
A00002-5-HRP

100 μg HRP conjugated

$570
A00002-5-APC

100 μg APC conjugated

$670
A00002-5-PE

100 μg PE conjugated

$670
A00002-5-carrier-free

Carrier Free

$370

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A00002-5
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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