Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody

TNF-alpha antibody

Boster Bio Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody (Catalog # M00002). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish. Cited in 10 publication(s).

Product Info Summary

SKU: M00002
Size: 100ug/vial
Reactive Species: Dog, Human, Mouse, Rabbit, Rat, Zebrafish, Cat
Host: Mouse
Application: IHC

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Product Name

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody

View all TNF-alpha Antibodies

SKU/Catalog Number

M00002

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody (Catalog # M00002). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00002)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: TNF706 + P/T2

Isotype

IgM, kappa

Immunogen

Recombinant N-terminal fragment of human TNF- (TNF706); Peptide corresponding to aa115-130 of human TNF- (P/T2)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00002 is reactive to TNF in Dog, Human, Mouse, Rabbit, Rat, Zebrafish, Cat

Applications

M00002 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

25.644kDa

Background of TNF-alpha

TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TNF (Source: Uniprot.org, NCBI)

Gene Name

TNF

Full Name

Tumor necrosis factor

Weight

25.644kDa

Superfamily

Tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TNF, check out the TNF Infographic

TNF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00002 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

TLR4 signaling is activated in ventilator-induced diaphragm dysfunction in rats

Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus

Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways

Sophocarpine Alleviates Injury-Induced Intima Hyperplasia of Carotid Arteries by Suppressing Inflammation in a Rat Model

Bone Marrow-Derived Mesenchymal Stem Cells Modified with Akt1 Ameliorates Acute Liver GVHD

Oxamate Enhances the Anti-Inflammatory and Insulin-Sensitizing Effects of Metformin in Diabetic Mice

Human umbilical cord‐derived mesenchymal stem cells conditioned medium attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells in an irreversible model of unilateral ureteral obstruction

Human Exosomes Accelerate Cutaneous Wound Healing by Promoting Collagen Synthesis in A Diabetic Mice Model

Lonicera japonica polysaccharides attenuate ovalbumin-induced allergic rhinitis by regulation of Th17 cells in BALB/c mice

Effect of curcumin on expressions of NF-κBp65, TNF-α and IL-8 in placental tissue of premature birth of infected mice

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3 Customer Q&As for Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody

Question

Our lab were happy with the WB result of your anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

According to literature, leukocyte does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2020-04-09

Question

We are currently using anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody M00002 for rabbit tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says canine, human, mouse, rabbit, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

The anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody (M00002) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-11

Question

We have been able to see staining in human prostatic carcinoma. Are there any suggestions? Is anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody supposed to stain prostatic carcinoma positively?

Verified Customer

Verified customer

Asked: 2018-07-19

Answer

From literature prostatic carcinoma does express TNF. From Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2018-07-19

Order DetailsPrice
M00002-100ug

100 μg

$468

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