Product Info Summary
SKU: | M00002 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Dog, Human, Mouse, Rabbit, Rat, Zebrafish, Cat |
Host: | Mouse |
Application: | IHC |
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Product info
Product Name
Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody
SKU/Catalog Number
M00002
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody (Catalog # M00002). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00002)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: TNF706 + P/T2
Isotype
IgM, kappa
Immunogen
Recombinant N-terminal fragment of human TNF- (TNF706); Peptide corresponding to aa115-130 of human TNF- (P/T2)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00002 is reactive to TNF in Dog, Human, Mouse, Rabbit, Rat, Zebrafish, Cat
Applications
M00002 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
25.644kDa
Background of TNF-alpha
TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Erdheim-Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNF706 + P/T2).
Protein Target Info & Infographic
Gene/Protein Information For TNF (Source: Uniprot.org, NCBI)
Gene Name
TNF
Full Name
Tumor necrosis factor
Weight
25.644kDa
Superfamily
Tumor necrosis factor family
Alternative Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNF, check out the TNF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody (M00002)
Hello CJ!
M00002 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
TLR4 signaling is activated in ventilator-induced diaphragm dysfunction in rats
Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus
Protection of Anthocyanin from Myrica rubra against Cerebral Ischemia-Reperfusion Injury via Modulation of the TLR4/NF-κB and NLRP3 Pathways
Sophocarpine Alleviates Injury-Induced Intima Hyperplasia of Carotid Arteries by Suppressing Inflammation in a Rat Model
Bone Marrow-Derived Mesenchymal Stem Cells Modified with Akt1 Ameliorates Acute Liver GVHD
Oxamate Enhances the Anti-Inflammatory and Insulin-Sensitizing Effects of Metformin in Diabetic Mice
Human umbilical cord‐derived mesenchymal stem cells conditioned medium attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells in an irreversible model of unilateral ureteral obstruction
Human Exosomes Accelerate Cutaneous Wound Healing by Promoting Collagen Synthesis in A Diabetic Mice Model
Lonicera japonica polysaccharides attenuate ovalbumin-induced allergic rhinitis by regulation of Th17 cells in BALB/c mice
Effect of curcumin on expressions of NF-κBp65, TNF-α and IL-8 in placental tissue of premature birth of infected mice
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody
Question
Our lab were happy with the WB result of your anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
According to literature, leukocyte does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody M00002 for rabbit tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says canine, human, mouse, rabbit, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody (M00002) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
We have been able to see staining in human prostatic carcinoma. Are there any suggestions? Is anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal antibody supposed to stain prostatic carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
From literature prostatic carcinoma does express TNF. From Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2018-07-19