Anti-TNNT3 Antibody Picoband™

TNNT3 antibody

Boster Bio Anti-TNNT3 Antibody Picoband™ catalog # A05885-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05885-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-TNNT3 Antibody Picoband™

View all TNNT3 Antibodies

SKU/Catalog Number

A05885-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TNNT3 Antibody Picoband™ catalog # A05885-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNNT3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05885-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TNNT3 recombinant protein (Position: A45-K251).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05885-2 is reactive to TNNT3 in Human, Mouse, Rat

Applications

A05885-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

31.825kDa

Background of TNNT3

Fast skeletal muscle troponin T (fTnT) is a protein that in humans is encoded by the TNNT3 gene. It is mapped to 11p15.5. The binding of Ca (2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca (2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca (2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca (2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For TNNT3 (Source: Uniprot.org, NCBI)

Gene Name

TNNT3

Full Name

Troponin T, fast skeletal muscle

Weight

31.825kDa

Superfamily

troponin T family

Alternative Names

Beta-TnTF; fast skeletal muscle; TnTf; troponin T type 3 (skeletal, fast); troponin T3, skeletal, fast; troponin-T3, skeletal, fast TNNT3 DA2B2, TNTF, beta-TnTF troponin T3, fast skeletal type troponin T, fast skeletal muscle|troponin T type 3 (skeletal, fast)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TNNT3, check out the TNNT3 Infographic

TNNT3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TNNT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-TNNT3 Antibody Picoband™

Question

We are currently using anti-TNNT3 antibody A05885-2 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

The anti-TNNT3 antibody (A05885-2) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-28

Order DetailsPrice
A05885-2

100μg

$370
A05885-2-10ug

10μg sample (liquid)

$99
A05885-2-Biotin

100 μg Biotin conjugated

$570
A05885-2-Cy3

100 μg Cy3 conjugated

$570
A05885-2-Dylight488

100 μg Dylight488 conjugated

$570
A05885-2-Dylight550

100 μg Dylight550 conjugated

$570
A05885-2-Dylight594

100 μg Dylight594 conjugated

$570
A05885-2-FITC

100 μg FITC conjugated

$570
A05885-2-HRP

100 μg HRP conjugated

$570
A05885-2-APC

100 μg APC conjugated

$670
A05885-2-PE

100 μg PE conjugated

$670
A05885-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A05885-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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