Click image to see more details
Product Info Summary
| SKU: | F00953-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Topo II alpha Antibody
SKU/Catalog Number
F00953-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Topo II alpha Antibody catalog # F00953-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topo II alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # F00953-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Isotype
IgG
Reactive Species
F00953-1 is reactive to TOP2A in Human, Mouse
Applications
F00953-1 is guaranteed for WB Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of extracts from K562 cells, using Topo IIalpha Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For TOP2A (Source: Uniprot.Org, NCBI)
Uniprot ID
P11388
Gene ID
7153
Gene Name
TOP2A
Full Name
DNA topoisomerase 2-alpha
Weight
174.385kDa
Superfamily
type II topoisomerase family
Alternative Names
DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; TOP2; TOP2A; TOP2DNA gyrase; topoisomerase (DNA) II alpha (170kD); topoisomerase (DNA) II alpha 170kDa; TP2A TOP2A TOP2, TP2A DNA topoisomerase II alpha DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha 170kDa
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TOP2A, check out the TOP2A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TOP2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Topo II alpha Antibody (F00953-1)
No publications found for F00953-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Anti-Topo II alpha Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovarian carcinoma using anti-Topo II alpha antibody F00953-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-07
Question
I was wanting to use your anti-Topo II alpha antibody for WB for human ovarian carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
You can see on the product datasheet, F00953-1 anti-Topo II alpha antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-19
Question
Does anti-Topo II alpha antibody F00953-1 work for WB with ovarian carcinoma?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
According to the expression profile of ovarian carcinoma, TOP2A is highly expressed in ovarian carcinoma. So, it is likely that anti-Topo II alpha antibody F00953-1 will work for WB with ovarian carcinoma.
Boster Scientific Support
Answered: 2020-01-17
Question
Will F00953-1 anti-Topo II alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
It shows on the product datasheet, F00953-1 anti-Topo II alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-18
Question
My question regarding product F00953-1, anti-Topo II alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free F00953-1 anti-Topo II alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-23
Question
I see that the anti-Topo II alpha antibody F00953-1 works with WB, what is the protocol used to produce the result images on the product page?
K. Li
Verified customer
Asked: 2019-04-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-29
Question
We want to test anti-Topo II alpha antibody F00953-1 on human ovarian carcinoma for research purposes, then I may be interested in using anti-Topo II alpha antibody F00953-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Kulkarni
Verified customer
Asked: 2018-06-06
Answer
The products we sell, including anti-Topo II alpha antibody F00953-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-06
Question
See below the WB image, lot number and protocol we used for ovarian carcinoma using anti-Topo II alpha antibody F00953-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-17
Question
Is a blocking peptide available for product anti-Topo II alpha antibody (F00953-1)?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
We do provide the blocking peptide for product anti-Topo II alpha antibody (F00953-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-08
Question
Do you have a BSA free version of anti-Topo II alpha antibody F00953-1 available?
B. Wu
Verified customer
Asked: 2016-10-05
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Topo II alpha antibody F00953-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-10-05
