SKU A07881-1
Size 100 ul
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Applications WB

Overview

Product Name Anti-TP53INP2 Antibody
SKU/Catalog Number A07881-1
Storage & Handling -20°C
Size 100 ul
Description Anti-TP53INP2 Antibody tested in WB|WB, reactive to human|mouse|rat
Cite This Product Anti-TP53INP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07881-1)
Host Rabbit
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human TP53INP2
Reactivity Human, Mouse, Rat

Assay Details

Images And Assay Conditions

/a/0/a07881 1 wb anti tp53inp2 antibody figure 1.jpg

Figure 1. Western blot analysis of TP53INP2 using anti-TP53INP2 antibody (A07881-1).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human A549 Whole Cell

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53INP2 antigen affinity purified polyclonal antibody (Catalog # A07881-1) at 1ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53INP2. The expected band size for TP53INP2 is at 23.9KD.

/a/0/a07881 1 wb anti tp53inp2 antibody figure 2.jpg

Figure 2. Western blot analysis of TP53INP2 using anti-TP53INP2 antibody (A07881-1).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human heart

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53INP2 antigen affinity purified polyclonal antibody (Catalog # A07881-1) at 0.2-1 ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53INP2. The expected band size for TP53INP2 is at 23.9KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q8IXH6
Gene Name TP53INP2
Protein Name Tumor protein p53-inducible nuclear protein 2
Alternative Names Tumor protein p53-inducible nuclear protein 2;Diabetes and obesity-regulated gene;p53-inducible protein U;PIG-U;TP53INP2;C20orf110, DOR, PINH;
Subcellular Localization Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation.
Molecular Weight 23980 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Dual regulator of transcription and autophagy. Positively regulates autophagy and is required for autophagosome formation and processing. May act as a scaffold protein that recruits MAP1LC3A, GABARAP and GABARAPL2 and brings them to the autophagosome membrane by interacting with VMP1 where, in cooperation with the BECN1-PI3-kinase class III complex, they trigger autophagosome development. Acts as a transcriptional activator of THRA.
Research Areas Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Intracellular, Oncoproteins/Suppressors, P53 Pathway, Tumor Suppressors

*You can search these to find other products in these research areas.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
1 Unit $299

USD $299

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