|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-TRAF1 Antibody|
|Description||Rabbit IgG polyclonal antibody for TNF receptor-associated factor 1(TRAF1) detection. Tested with WB in Human;Mouse.|
|Cite This Product||Anti-TRAF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2006)|
|Replacement Item||This antibody may replace the following items: sc-6253|sc-7186|sc-1831|sc-1830|sc-874|sc-271683|sc-983|sc-514936|sc-515161 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human TRAF1(220-235aa HQSQLDRERILSLEQR), different from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||TNF receptor-associated factor 1|
|Molecular Weight||46164 MW|
|Protein Function||Adapter molecule that regulates the activation of NF- kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein- ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. .|
|Sequence Similarities||Contains 1 MATH domain.|
|Alternative Names||TNF receptor-associated factor 1;Epstein-Barr virus-induced protein 6;TRAF1;EBI6;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases| microbiology|interspecies interaction|host virus interaction| signal transduction|signaling pathway|nuclear signaling|nfkb pathway|growth factors/hormones|tnf| epigenetics and nuclear signaling|nuclear signaling pathways| cancer|growth factors||
Background for TNF receptor-associated factor 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TRAF1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TRAF1 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF1, 30.6KD (162aa tag+ E174-E282)
Lane 1: Recombinant Human TRAF1 Protein 5ng
Lane 2: Recombinant Human TRAF1 Protein 2.5ng
Lane 3: Recombinant Human TRAF1 Protein 1.25ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,