|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-TRAF2 Antibody|
|Description||Rabbit IgG polyclonal antibody for TNF receptor-associated factor 2(TRAF2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-TRAF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2007)|
|Replacement Item||This antibody may replace the following items: sc-877|sc-7187|sc-7346|sc-136998|sc-137048|sc-136999|sc-136997|sc-365287|sc-876 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human TRAF2(305-325aa RQHRLDQDKIEALSSKVQQLE), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||TNF receptor-associated factor 2|
|Molecular Weight||55859 MW|
|Protein Function||Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. .|
|Sequence Similarities||Belongs to the TNF receptor-associated factor family. A subfamily.|
|Subcellular Localization||Cytoplasm .|
|Alternative Names||TNF receptor-associated factor 2;6.3.2.-;E3 ubiquitin-protein ligase TRAF2;Tumor necrosis factor type 2 receptor-associated protein 3;TRAF2;TRAP3;|
|Research Areas|||cell biology|apoptosis|intracellular|associated proteins| signal transduction|adapters|cytoplasmic|signaling pathway|nuclear signaling|nfkb pathway|growth factors/hormones|tnf| cancer|growth factors| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators||
Background for TNF receptor-associated factor 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TRAF2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TRAF2 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF2, 30.4KD (162aa tag+ Q261-V369)
Lane 1: Recombinant Human TRAF2 Protein 10ng
Lane 2: Recombinant Human TRAF2 Protein 5ng
Lane 3: Recombinant Human TRAF2 Protein 2.5ng
IHC(P): Human Tonsil Tissue
ICC: HELA Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,