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SKU:PA2008
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-TRAF3 Antibody
SKU/Catalog Number PA2008
Description Rabbit IgG polyclonal antibody for TNF receptor-associated factor 3(TRAF3) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-TRAF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2008)
Replacement Item This antibody may replace the following items: sc-1574|sc-948|sc-1828|sc-6933|sc-949|sc-947|sc-948-G|sc-947-G from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human TRAF3(387-403aa RHDQMLSVHDIRLADMD), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TRAF3
Protein Name TNF receptor-associated factor 3
Molecular Weight 64490 MW
Protein Function Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. .
Sequence Similarities Belongs to the TNF receptor-associated factor family. A subfamily.
Subcellular Localization Cytoplasm . Endosome . Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus. .
Uniprot ID Q13114
Alternative Names TNF receptor-associated factor 3;6.3.2.-;CAP-1;CD40 receptor-associated factor 1;CRAF1;CD40-binding protein;CD40BP;LMP1-associated protein 1;LAP1;TRAF3;CAP1, CRAF1;
Research Areas |immunology|innate immunity|cytokines|tnf superfamily| signal transduction|growth factors/hormones|tnf| cancer|growth factors|invasion/microenvironment|apoptosis|death receptors & ligands|traf|cell death|receptors|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for TNF receptor-associated factor 3

TRAF3(TNF Receptor-Associated Factor 3), also called LAP1, CAP1, CRAF1 or CD40BP, is a protein that in humans is encoded by the TRAF3 gene. The protein encoded by this gene is a member of the TNF receptor(TNFR) associated factor(TRAF) protein family. Gross(2012) mapped the TRAF3 gene to chromosome 14q32.32 based on an alignment of the TRAF3 sequence with the genomic sequence(GRCh37). Dadgostar et al.(2003) determined that the coiled-coil domain of mouse T3jam interacted with the isoleucine zipper domain of Traf3. Oganesyan et al.(2006) demonstrated that cells lacking TRAF3 are defective in type I interferon responses activated by several different Toll-like receptors.

Anti-TRAF3 Antibody Images

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Anti-TRAF3 Antibody
Anti-TRAF3 antibody, PA2008, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF3, 30.6KD (162aa tag+ R341-Y449)
Lane 1: Recombinant Human TRAF3 Protein 5ng
Lane 2: Recombinant Human TRAF3 Protein 2.5ng
Lane 3: Recombinant Human TRAF3 Protein 1.25ng
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lap1 antibody, cap1 antibody, traf3 antibody