Anti-TRAF4 Rabbit Monoclonal Antibody

TRAF-4 antibody

Boster Bio Anti-TRAF4 Rabbit Monoclonal Antibody catalog # M03069. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M03069
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-TRAF4 Rabbit Monoclonal Antibody

View all TRAF-4 Antibodies

SKU/Catalog Number

M03069

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TRAF4 Rabbit Monoclonal Antibody catalog # M03069. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRAF4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03069)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BGH-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TRAF4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M03069 is reactive to TRAF4 in Human, Mouse

Applications

M03069 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

53.543kDa

Background of TRAF-4

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For TRAF4 (Source: Uniprot.org, NCBI)

Gene Name

TRAF4

Full Name

TNF receptor-associated factor 4

Weight

53.543kDa

Superfamily

TNF receptor-associated factor family

Alternative Names

CART1; CART1RING finger protein 83; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; MLN62; MLN62cysteine-rich domain associated with ring and TRAF domain; RNF83malignant 62; TNF receptor-associated factor 4; TRAF4; TRAF-4; tumor necrosis receptor-associated factor 4A TRAF4 CART1, MLN62, RNF83 TNF receptor associated factor 4 TNF receptor-associated factor 4|MLN 62|RING finger protein 83|TRAF4 variant 6|cysteine-rich domain associated with RING and Traf domains protein 1|cysteine-rich domain associated with ring and TRAF domain|malignant 62|metastatic lymph node gene 62 protein|tumor necrosis receptor-associated factor 4A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRAF4, check out the TRAF4 Infographic

TRAF4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M03069

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-TRAF4 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-TRAF4 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-TRAF4 Rabbit Monoclonal Antibody

Question

Why can't we get a signal when using M03069 on HELA and liver tissue WB?

Verified customer

Asked: 2023-01-06

Answer

Please see the following tips to improve test result using the Anti-TRAF4 Rabbit Monoclonal Antibody (M03069): 1. Please use lysis buffer with 1%SDS if the LDS lysis buffer is a non-reducing buffer. 2. The membrane cutting area should preferably be within 20-80kDa. Place 50kDa in the middle of PVDF membrane. 3. Load equal amounts (20-30 ug) of protein into each well of the SDS-PAGE gel, along with appropriate molecular weight marker. 1 ug is too little. 4. Block the membrane for 1 hour at room temperature using 1%BSA/TBST or 2-3% non-fat Milk/ TBST. Dilute the antibodies for 1 hour at room temperature using 1%BSA/TBST or 2-3% non-fat Milk/ TBST. 5. Increase the concentration of the primary antibody and the secondary antibody.e.g. primary antibody 1:500-1000, secondary antibody 1:1000-2000. 6. Do not overuse ECL substrate. After substrate incubation, remove the excess ECL substrate from the membrane before exposure (place the membrane vertically, and the ECL substrate liquid does not flow down, and then use paper to absorb the excess ECL substrate around the membrane). This can prevent the flow of ECL substrate from causing strong non-specific bands, dark background and irregular black marks.

Boster Scientific Support

Answered: 2023-01-07

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-TRAF4 Rabbit Monoclonal antibody M03069. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-06-28

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-28

Question

Is this M03069 anti-TRAF4 Rabbit Monoclonal antibody reactive to the isotypes of TRAF4?

Verified Customer

Verified customer

Asked: 2018-05-16

Answer

The immunogen of M03069 anti-TRAF4 Rabbit Monoclonal antibody is A synthesized peptide derived from human TRAF4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-16

Question

Would anti-TRAF4 Rabbit Monoclonal antibody M03069 work for WB with lung?

J. Walker

Verified customer

Asked: 2018-02-09

Answer

According to the expression profile of lung, TRAF4 is highly expressed in lung. So, it is likely that anti-TRAF4 Rabbit Monoclonal antibody M03069 will work for WB with lung.

Boster Scientific Support

Answered: 2018-02-09

Question

I was wanting to use your anti-TRAF4 Rabbit Monoclonal antibody for WB for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?

D. Banerjee

Verified customer

Asked: 2015-10-05

Answer

It shows on the product datasheet, M03069 anti-TRAF4 Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-10-05

Order DetailsPrice
M03069

100μL

$370
M03069-30ul

30μL sample

$99
M03069-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M03069
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.