Anti-TRAM1 Antibody

TRAM1 antibody

Boster Bio Anti-TRAM1 Antibody catalog # PA2010. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2010
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, IHC-F, ICC, WB

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Product Name

Anti-TRAM1 Antibody

View all TRAM1 Antibodies

SKU/Catalog Number

PA2010

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRAM1 Antibody catalog # PA2010. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRAM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2010)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2010 is reactive to TRAM1 in Human, Mouse, Rat

Applications

PA2010 is guaranteed for IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

43.072kDa

Background of TRAM1

TRAM1 (Translocation-Associating Membrane Protein 1), By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al. (1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the TRAM gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For TRAM1 (Source: Uniprot.org, NCBI)

Gene Name

TRAM1

Full Name

Translocating chain-associated membrane protein 1

Weight

43.072kDa

Superfamily

TRAM family

Alternative Names

PNAS8; PRO1292; TRAMP; TRAMtranslocating chain-associated membrane protein 1; translocating chain-associating membrane protein; translocation associated membrane protein 1; translocation-associating membrane protein 1 TRAM1 PNAS8, TRAM, TRAMP translocation associated membrane protein 1 translocating chain-associated membrane protein 1|translocating chain-associating membrane protein|translocation-associating membrane protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRAM1, check out the TRAM1 Infographic

TRAM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-TRAM1 Antibody

Question

Will anti-TRAM1 antibody PA2010 work for IHC-P with choroid plexus epithelium?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

According to the expression profile of choroid plexus epithelium, TRAM1 is highly expressed in choroid plexus epithelium. So, it is likely that anti-TRAM1 antibody PA2010 will work for IHC-P with choroid plexus epithelium.

Boster Scientific Support

Answered: 2019-11-13

Question

We are currently using anti-TRAM1 antibody PA2010 for mouse tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The anti-TRAM1 antibody (PA2010) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-15

Question

I was wanting to use your anti-TRAM1 antibody for IHC-P for mouse choroid plexus epithelium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse choroid plexus epithelium identification?

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

You can see on the product datasheet, PA2010 anti-TRAM1 antibody has been tested for IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse choroid plexus epithelium in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-30

Question

Is this PA2010 anti-TRAM1 antibody reactive to the isotypes of TRAM1?

Verified Customer

Verified customer

Asked: 2019-01-21

Answer

The immunogen of PA2010 anti-TRAM1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TRAM1(314-331aa KFINFQLRRWREHSAFQA), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-21

Question

Can you help my question with product PA2010, anti-TRAM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-07-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2010 anti-TRAM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-07-19

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for choroid plexus epithelium using anti-TRAM1 antibody PA2010. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-29

Order DetailsPrice
PA2010

100μg

$370
PA2010-10ug

10μg sample (liquid)

$99
PA2010-Biotin

100 μg Biotin conjugated

$570
PA2010-Cy3

100 μg Cy3 conjugated

$570
PA2010-Dylight488

100 μg Dylight488 conjugated

$570
PA2010-Dylight550

100 μg Dylight550 conjugated

$570
PA2010-Dylight594

100 μg Dylight594 conjugated

$570
PA2010-FITC

100 μg FITC conjugated

$570
PA2010-HRP

100 μg HRP conjugated

$570
PA2010-APC

100 μg APC conjugated

$670
PA2010-PE

100 μg PE conjugated

$670
PA2010-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2010
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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