Anti-TRFP/MED20 Antibody Picoband™

MED20 antibody

Boster Bio Anti-TRFP/MED20 Antibody Picoband™ catalog # A09407-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A09407-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-TRFP/MED20 Antibody Picoband™

View all MED20 Antibodies

SKU/Catalog Number

A09407-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRFP/MED20 Antibody Picoband™ catalog # A09407-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRFP/MED20 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09407-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human TRFP / MED20 recombinant protein (Position: M1-R212).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09407-1 is reactive to MED20 in Human, Mouse, Rat

Applications

A09407-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

23.222kDa

Background of MED20

MED20 is a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For MED20 (Source: Uniprot.org, NCBI)

Gene Name

MED20

Full Name

Mediator of RNA polymerase II transcription subunit 20

Weight

23.222kDa

Superfamily

Mediator complex subunit 20 family

Alternative Names

hTRFP; mediator complex subunit 20MGC29869; mediator of RNA polymerase II transcription subunit 20; PRO0213; Trf (TATA binding protein-related factor)-proximal homolog (Drosophila); Trf (TATA binding protein-related factor)-proximal homolog; TRFPDKFZp586D2223; TRF-proximal protein homolog MED20 PRO0213, SRB2, TRFP mediator complex subunit 20 mediator of RNA polymerase II transcription subunit 20|Trf (TATA binding protein-related factor)-proximal homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MED20, check out the MED20 Infographic

MED20 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MED20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-TRFP/MED20 Antibody Picoband™

Question

We are currently using anti-TRFP/MED20 antibody A09407-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

The anti-TRFP/MED20 antibody (A09407-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-05

Order DetailsPrice
A09407-1

100μg

$370
A09407-1-10ug

10μg sample (liquid)

$99
A09407-1-Biotin

100 μg Biotin conjugated

$570
A09407-1-Cy3

100 μg Cy3 conjugated

$570
A09407-1-Dylight488

100 μg Dylight488 conjugated

$570
A09407-1-Dylight550

100 μg Dylight550 conjugated

$570
A09407-1-Dylight594

100 μg Dylight594 conjugated

$570
A09407-1-FITC

100 μg FITC conjugated

$570
A09407-1-HRP

100 μg HRP conjugated

$570
A09407-1-APC

100 μg APC conjugated

$670
A09407-1-PE

100 μg PE conjugated

$670
A09407-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A09407-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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