Anti-TRIM24/Tif1 Alpha Antibody

Boster Bio Anti-TRIM24/Tif1 Alpha Antibody catalog # A03258-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A03258-1
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-TRIM24/Tif1 Alpha Antibody

See all TIF1 alpha products

SKU/Catalog Number

A03258-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-TRIM24/Tif1 Alpha Antibody catalog # A03258-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRIM24/Tif1 Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03258-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from C-terminal of human TRIM24.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03258-1 is reactive to TRIM24 in Human, Mouse

Applications

A03258-1 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
IHC, 1:50-1:100

Validation Images & Assay Conditions

Gene/Protein Information For TRIM24 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15164

Gene ID

8805

Gene Name

TRIM24

Full Name

Transcription intermediary factor 1-alpha

Weight

116.831kDa

Alternative Names

E3 ubiquitin-protein ligase TRIM24; EC 6.3.2; EC 6.3.2.-; hTIF1; PTC6; RING finger protein 82; RNF82Tif1a; TIF1-alpha; TIF1ATIF1ALPHA; TIF1TF1A; transcription intermediary factor 1-alpha; transcriptional intermediary factor 1; tripartite motif containing 24; tripartite motif-containing 24; Tripartite motif-containing protein 24 TRIM24 PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1 tripartite motif containing 24 transcription intermediary factor 1-alpha|E3 ubiquitin-protein ligase TRIM24|RING finger protein 82|RING-type E3 ubiquitin transferase TIF1-alpha|TIF1-alpha|transcriptional intermediary factor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TRIM24, check out the TRIM24 Infographic

TRIM24 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TRIM24: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-TRIM24/Tif1 Alpha Antibody

Question

Does A03258-1 anti-TRIM24/Tif1 Alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

As indicated on the product datasheet, A03258-1 anti-TRIM24/Tif1 Alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-11

Question

Here is the WB image, lot number and protocol we used for sperm using anti-TRIM24/Tif1 Alpha antibody A03258-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-09

Question

We are currently using anti-TRIM24/Tif1 Alpha antibody A03258-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-17

Answer

The anti-TRIM24/Tif1 Alpha antibody (A03258-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-17

Question

Is this A03258-1 anti-TRIM24/Tif1 Alpha antibody reactive to the isotypes of TRIM24?

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

The immunogen of A03258-1 anti-TRIM24/Tif1 Alpha antibody is Synthesized peptide derived from C-terminal of human TRIM24. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-26

Question

I was wanting to use your anti-TRIM24/Tif1 Alpha antibody for WB for mouse sperm on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse sperm identification?

Verified Customer

Verified customer

Asked: 2017-08-14

Answer

It shows on the product datasheet, A03258-1 anti-TRIM24/Tif1 Alpha antibody has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse sperm in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-14

Question

Will anti-TRIM24/Tif1 Alpha antibody A03258-1 work for WB with sperm?

J. Li

Verified customer

Asked: 2013-11-28

Answer

According to the expression profile of sperm, TRIM24 is highly expressed in sperm. So, it is likely that anti-TRIM24/Tif1 Alpha antibody A03258-1 will work for WB with sperm.

Boster Scientific Support

Answered: 2013-11-28

Question

Does anti-TRIM24/Tif1 Alpha antibody A03258-1 work on feline WB with liver?

A. Patel

Verified customer

Asked: 2013-02-05

Answer

Our lab technicians have not tested anti-TRIM24/Tif1 Alpha antibody A03258-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-TRIM24/Tif1 Alpha antibody A03258-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-02-05

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A03258-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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