Anti-TRIM25 Monoclonal Antibody

TRIM25 antibody

Boster Bio Anti-TRIM25 Monoclonal Antibody catalog # M03232. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M03232
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-TRIM25 Monoclonal Antibody

View all TRIM25 Antibodies

SKU/Catalog Number

M03232

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TRIM25 Monoclonal Antibody catalog # M03232. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRIM25 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03232)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADFG-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M03232 is reactive to TRIM25 in Human, Mouse, Rat

Applications

M03232 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

71 kDa

Calculated molecular weight

70.973kDa

Background of TRIM25

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:60

Validation Images & Assay Conditions

Gene/Protein Information For TRIM25 (Source: Uniprot.org, NCBI)

Gene Name

TRIM25

Full Name

E3 ubiquitin/ISG15 ligase TRIM25

Weight

70.973kDa

Alternative Names

EC 6.3.2.19; EC 6.3.2.n3; EFPZ147; Estrogen-responsive finger protein; RING finger protein 147; RNF147E3 ubiquitin/ISG15 ligase TRIM25; tripartite motif containing 25; tripartite motif-containing 25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147 (estrogen-responsive finger protein); Zinc finger protein 147; zinc finger protein-147; ZNF147tripartite motif protein TRIM25 TRIM25 EFP, RNF147, Z147, ZNF147 tripartite motif containing 25 E3 ubiquitin/ISG15 ligase TRIM25|RING finger protein 147|RING-type E3 ubiquitin transferase TRIM25|estrogen-responsive finger protein|tripartite motif protein TRIM25|tripartite motif-containing protein 25|ubiquitin/ISG15-conjugating enzyme TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRIM25, check out the TRIM25 Infographic

TRIM25 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRIM25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-TRIM25 Monoclonal Antibody

Question

Would anti-TRIM25 Monoclonal antibody M03232 work for IP with placenta?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

According to the expression profile of placenta, TRIM25 is highly expressed in placenta. So, it is likely that anti-TRIM25 Monoclonal antibody M03232 will work for IP with placenta.

Boster Scientific Support

Answered: 2020-03-18

Question

Would anti-TRIM25 Monoclonal antibody M03232 work on primate IP with liver?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

Our lab technicians have not validated anti-TRIM25 Monoclonal antibody M03232 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TRIM25 Monoclonal antibody M03232 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-06

Question

We are currently using anti-TRIM25 Monoclonal antibody M03232 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

The anti-TRIM25 Monoclonal antibody (M03232) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-09

Question

Is this M03232 anti-TRIM25 Monoclonal antibody reactive to the isotypes of TRIM25?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

The immunogen of M03232 anti-TRIM25 Monoclonal antibody is A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-01

Question

Do you have a BSA free version of anti-TRIM25 Monoclonal antibody M03232 available?

G. Carter

Verified customer

Asked: 2019-05-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRIM25 Monoclonal antibody M03232 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-03

Question

Please see the WB image, lot number and protocol we used for placenta using anti-TRIM25 Monoclonal antibody M03232. Please let me know if you require anything else.

O. Huang

Verified customer

Asked: 2018-07-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-23

Order DetailsPrice
M03232

100μL

$370
M03232-30ul

30μL sample

$99
M03232-carrier-free

Carrier Free

$370

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M03232
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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