|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-TrkA/NTRK1 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for High affinity nerve growth factor receptor(NTRK1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-TrkA/NTRK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1321)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human TrkA(342-361aa RHGCLRLNQPTHVNNGNYTL), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-TrkA antibody, PA1321, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: SHG Cell Lysate
Lane 5: NEURO Cell Lysate
Anti-TrkA antibody, PA1321, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||High affinity nerve growth factor receptor|
|Tissue Specificity||Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. .|
|Alternative Names||High affinity nerve growth factor receptor;184.108.40.206;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;Trk-A;gp140trk;p140-TrkA;NTRK1;MTC, TRK, TRKA;|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). .|
|Molecular Weight||87497 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras- PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||Trk A(Neurotrophic tyrosine kinase receptor A) is the high affinity catalytic receptor for the neurotrophin, Nerve Growth Factor(NGF). Higher affinity binding of NGFR can achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA(NTRK1), TRKB(NTRK2), and TRKC(NTRK3). TRKA, TRKB, and TRKC are specific for or "preferred by" NGF, NTF4(Neurotrophin-4) and BDNF, and NTF3(Neurotrophin-3), respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity. The absence of TrkA(NGFR) expression was associated with a strong increase in the Sp3 repressor short isoform(s) and a lack of the Sp3 activator long isoform. Sp3 is a bifunctional transcription factor that has been reported to stimulate or repress the transcription of numerous genes. Indo et al.(1996) concluded that defects in TRKA cause CIPA(Congenital insensitivity to pain with anhidrosis) and that the NGF-TRKA system has a crucial role in the development and function of the nociceptive reception as well as establishment of thermoregulation via sweating in humans. These results also implicate genes encoding other TRK and neurotrophin family members as candidates for developmental defect(s) of the nervous system.|
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at email@example.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: I have found one study used the antibody from your company (Anti-TrkA Antibody PA1321). So could you please give me more information about the antibody regarding the dilution for IHC and antigen retrieval as it was different in the previous study than the recommended in antibody datasheet. also if there is any published data in cancer specially head and neck cancer.A: The recommended concentration is 0.5-1μg/ml for IHC-P. The final optimal dilution ratio should be determined by the users themselves as each experiment is different. In our initial research we were not able to find any publications specific to cancer in the head or neck area.
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?A: One other very common name is trk antibody