| SKU: | MA1096 |
|---|---|
| Pack Size: | 100μg/vial |
| Validated Species: | Chicken, Human, Rabbit |
| Clonality: | Monoclonal |
| Clone Number: | TM228 |
| Host: | Mouse |
| Application: | IHC, WB |
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Overview
| Product Name | Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) |
|---|---|
| SKU/Catalog Number | MA1096 |
| Description | Mouse IgG monoclonal antibody for Tropomyosin (36kD) detection. Tested with WB, IHC-F in Human;rabbit;chicken. No cross reactivity with other proteins. |
| Cite This Product | Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) TM228 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096) |
| Host | Mouse |
| Validated Species | Chicken, Human, Rabbit |
| Predicted Species | Bovine *This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species. |
| Application | IHC, WB *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Recommended Detection Systems | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F). *Blocking peptide can be purchased at $50. Contact us for more information **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018! |
| Immunogen | Chicken gizzard tropomyosin. |
| Cross Reactivity | No cross reactivity with other proteins |
| Pack Size | 100μg/vial |
Properties
| Clonality | Monoclonal |
|---|---|
| Clone Number | TM228 |
| Form | Lyophilized |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Concentration | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Purification | Ascites |
| Isotype | Mouse IgG2a |
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | Tpm4 |
|---|---|
| Protein Name | Tropomyosin alpha-1 chain |
| Molecular Weight | 28510 MW |
| Protein Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium. |
| Tissue Specificity | TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle. |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Uniprot ID | P09495 |
| Alternative Names | Tropomyosin alpha-4 chain;Tropomyosin-4;TM-4;Tpm4; |
| Research Areas | |signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|tropomyosin| |
Background for Tropomyosin alpha-1 chain
The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.
Related Products
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Price: FREE if purchase with Primary antibody
Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)
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SuperVision IHC Kit with anti-Mouse IgG (SV0001-1)
Price: $150
Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) Images
Click the images to enlarge.
Western blot analysis of Tropomyosin(36kDa) expression in rat skeletal muscle extract (lane 1), rat cardiac muscle extract (lane 2), mouse skeletal muscle extract (lane 3), mouse cardiac muscle extract (lane 4), and HELA whole cell lysates (lane 5). Tropomyosin(36kDa) at 36KD was detected using rabbit anti-Tropomyosin(36kDa) Antigen Affinity purified polyclonal antibody (Catalog # MA1096) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
