Overview
| Product Name |
Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)
See more tropomyosin-4 products |
| Catalog# |
MA1096 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-Tropomyosin (36kD) Tpm4 Antibody (Monoclonal, TM228) catalog # MA1096. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Rabbit. |
| Cite This Product |
Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Mouse |
| Reactive Species |
Chicken, Human, Rabbit |
| Predicted Reactivity |
Bovine
*This antibody is predicted to react with the above species based on antigen
sequence similarities. Our Boster Guarantee covers the use of this product with the above species. |
| Applications |
IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
Chicken gizzard tropomyosin. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For Tpm4 (Source: Uniprot.org, NCBI)
| Uniprot Id | P09495 |
|---|
| NCBI Gene Id | 24852 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Tpm4 |
|---|
| Protein Name | Tropomyosin alpha-4 chain |
|---|
| Superfamily | tropomyosin family |
|---|
| Alternative Names | tropomyosin-4|TM30p1; tropomyosin 4; tropomyosin alpha-4 chain; tropomyosin-4 |
|---|
| Molecular Weight | 28510 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tpm4, check out the Tpm4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Tpm4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Tpm4 infographic
Our Boster Quality Guarantee for Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228) covers its use in the following applications.
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -
Western blot, 0.5-1μg/ml, Human, chicken, rabbit
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of Tropomyosin(36kDa) expression in rat skeletal muscle extract (lane 1), rat cardiac muscle extract (lane 2), mouse skeletal muscle extract (lane 3), mouse cardiac muscle extract (lane 4), and HELA whole cell lysates (lane 5). Tropomyosin(36kDa) at 36KD was detected using mouse anti-Tropomyosin(36kDa) Antigen Affinity purified monoclonal antibody (Catalog # MA1096) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Questions and answers from customers related to MA1096 Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)
6 Related Questions
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096. Let me know if you need anything else.
Verified Customer
Asked: 2019-11-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
We are currently using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 for rabbit tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says chicken, human, rabbit. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Asked: 2019-10-24
Answer
The anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) (MA1096) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-24
Question
Our lab want to know about to test anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 on chicken eye for research purposes, then I may be interested in using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2019-09-11
Answer
The products we sell, including anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-11
Question
Would anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 work for WB with eye?
T. Jones
Asked: 2019-02-04
Answer
According to the expression profile of eye, TPM4 is highly expressed in eye. So, it is likely that anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 will work for WB with eye.
Boster Scientific Support
Answered: 2019-02-04
Question
I have a question about product MA1096, anti-Tropomyosin(36kD) antibody (Monoclonal, TM228). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Johnson
Asked: 2018-06-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-12
Question
Will MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Answer
You can see on the product datasheet, MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-28
