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Product Info Summary
|Reactive Species:||Chicken, Human, Rabbit|
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Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)
Boster Bio Anti-Tropomyosin (36kD) Tpm4 Antibody (Monoclonal, TM228) catalog # MA1096. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Rabbit.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Chicken gizzard tropomyosin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1096 is reactive to Tpm4 in Chicken, Human, Rabbit
MA1096 is guaranteed for IHC, WB Boster Guarantee
Background of tropomyosin-4
The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -
Western blot, 0.5-1μg/ml, Human, chicken, rabbit
Validation Images & Assay Conditions
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Western blot analysis of Tropomyosin(36kDa) expression in rat skeletal muscle extract (lane 1), rat cardiac muscle extract (lane 2), mouse skeletal muscle extract (lane 3), mouse cardiac muscle extract (lane 4), and HELA whole cell lysates (lane 5). Tropomyosin(36kDa) at 36KD was detected using mouse anti-Tropomyosin(36kDa) Antigen Affinity purified monoclonal antibody (Catalog # MA1096) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Gene/Protein Information For Tpm4 (Source: Uniprot.Org, NCBI)
Tropomyosin alpha-4 chain
TM30p1; tropomyosin 4; tropomyosin alpha-4 chain; tropomyosin-4 Tpm4|Tpm4.2.2cy, Tpm4|tropomyosin 4|tropomyosin alpha-4 chain|TM-4|tropomycin 4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tpm4, check out the Tpm4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tpm4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for MA1096
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