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| SKU | MA1096 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Chicken, Human, Rabbit |
| Clonality | Monoclonal |
| Clone Number | TM228 |
| Host | Mouse |
| Ig Isotype | Mouse IgG2a |
| Applications | IHC, WB |
Overview
| Product Name | Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) |
|---|---|
| SKU/Catalog Number | MA1096 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for Tropomyosin (36kD) detection. Tested with WB, IHC-F in Human;rabbit;chicken. No cross reactivity with other proteins. |
| Cite This Product | Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Immunogen | Chicken gizzard tropomyosin. |
| Reactivity | Chicken, Human, Rabbit |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -
Western blot, 0.5-1μg/ml, Human, chicken, rabbit
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -
Western blot, 0.5-1μg/ml, Human, chicken, rabbit
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).Images And Assay Conditions
Western blot analysis of Tropomyosin(36kDa) expression in rat skeletal muscle extract (lane 1), rat cardiac muscle extract (lane 2), mouse skeletal muscle extract (lane 3), mouse cardiac muscle extract (lane 4), and HELA whole cell lysates (lane 5). Tropomyosin(36kDa) at 36KD was detected using rabbit anti-Tropomyosin(36kDa) Antigen Affinity purified polyclonal antibody (Catalog # MA1096) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P09495 |
|---|---|
| Gene Name | Tpm4 |
| Protein Name | Tropomyosin alpha-4 chain |
| Tissue Specificity | TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle. |
| Alternative Names | Tropomyosin alpha-4 chain;Tropomyosin-4;TM-4;Tpm4; |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Molecular Weight | 28510 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium. |
|---|---|
| Research Areas | Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction *You can search these to find other products in these research areas. |
| Background | The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells. |
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Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.