|Reactivity||Chicken, Human, Rabbit|
|Ig Isotype||Mouse IgG2a|
|Product Name||Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Tropomyosin (36kD) detection. Tested with WB, IHC-F in Human;rabbit;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Chicken gizzard tropomyosin.|
|Reactivity||Chicken, Human, Rabbit|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -
Western blot, 0.5-1μg/ml, Human, chicken, rabbit
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
Images And Assay Conditions
Western blot analysis of Tropomyosin(36kDa) expression in rat skeletal muscle extract (lane 1), rat cardiac muscle extract (lane 2), mouse skeletal muscle extract (lane 3), mouse cardiac muscle extract (lane 4), and HELA whole cell lysates (lane 5). Tropomyosin(36kDa) at 36KD was detected using rabbit anti-Tropomyosin(36kDa) Antigen Affinity purified polyclonal antibody (Catalog # MA1096) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info (Source: Uniprot.org)
|Protein Name||Tropomyosin alpha-4 chain|
|Tissue Specificity||TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.|
|Alternative Names||Tropomyosin alpha-4 chain;Tropomyosin-4;TM-4;Tpm4;|
|Subcellular Localization||Cytoplasm, cytoskeleton.|
|Molecular Weight||28510 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.|
|Research Areas||Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction
*You can search these to find other products in these research areas.
|Background||The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (MA1096)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.