|Validated Species:||Chicken, Human, Rabbit|
Data & Images
|Product Name||Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228)|
|Description||Mouse IgG monoclonal antibody for Tropomyosin (36kD) detection. Tested with WB, IHC-F in Human;rabbit;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) TM228 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096)|
|Validated Species||Chicken, Human, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Chicken gizzard tropomyosin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tropomyosin alpha-1 chain|
|Molecular Weight||28510 MW|
|Protein Function||Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.|
|Tissue Specificity||TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.|
|Subcellular Localization||Cytoplasm, cytoskeleton.|
|Alternative Names||Tropomyosin alpha-4 chain;Tropomyosin-4;TM-4;Tpm4;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|tropomyosin||
Background for Tropomyosin alpha-1 chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -|
Western blot, 0.5-1μg/ml, Human, chicken, rabbit
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Tropomyosin(36kD) Antibody (Monoclonal, TM228) Images
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