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Product Info Summary
| SKU: | MA1096 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Chicken, Human, Rabbit |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)
View all tropomyosin-4 Antibodies
SKU/Catalog Number
MA1096
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tropomyosin (36kD) Tpm4 Antibody (Monoclonal, TM228) catalog # MA1096. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Rabbit.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
TM228
Isotype
Mouse IgG2a
Immunogen
Chicken gizzard tropomyosin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
MA1096 is reactive to Tpm4 in Chicken, Human, Rabbit
Applications
MA1096 is guaranteed for IHC, WB Boster Guarantee
Background of tropomyosin-4
The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -
Western blot, 0.5-1μg/ml, Human, chicken, rabbit
Validation Images & Assay Conditions
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Western blot analysis of Tropomyosin(36kDa) expression in rat skeletal muscle extract (lane 1), rat cardiac muscle extract (lane 2), mouse skeletal muscle extract (lane 3), mouse cardiac muscle extract (lane 4), and HELA whole cell lysates (lane 5). Tropomyosin(36kDa) at 36KD was detected using mouse anti-Tropomyosin(36kDa) Antigen Affinity purified monoclonal antibody (Catalog # MA1096) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Protein Target Info & Infographic
Gene/Protein Information For Tpm4 (Source: Uniprot.Org, NCBI)
Uniprot ID
P09495
Gene ID
24852
Gene Name
Tpm4
Full Name
Tropomyosin alpha-4 chain
Weight
28.51kDa
Superfamily
tropomyosin family
Alternative Names
TM30p1; tropomyosin 4; tropomyosin alpha-4 chain; tropomyosin-4 Tpm4|Tpm4.2.2cy, Tpm4|tropomyosin 4|tropomyosin alpha-4 chain|TM-4|tropomycin 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tpm4, check out the Tpm4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tpm4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228) (MA1096)
No publications found for MA1096
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
We are currently using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 for rabbit tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says chicken, human, rabbit. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) (MA1096) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-24
Question
Our lab want to know about to test anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 on chicken eye for research purposes, then I may be interested in using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The products we sell, including anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-11
Question
Would anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 work for WB with eye?
T. Jones
Verified customer
Asked: 2019-02-04
Answer
According to the expression profile of eye, TPM4 is highly expressed in eye. So, it is likely that anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 will work for WB with eye.
Boster Scientific Support
Answered: 2019-02-04
Question
I have a question about product MA1096, anti-Tropomyosin(36kD) antibody (Monoclonal, TM228). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Johnson
Verified customer
Asked: 2018-06-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-12
Question
Will MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Wu
Verified customer
Asked: 2017-08-28
Answer
You can see on the product datasheet, MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-28
