Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)

Boster Bio Anti-Tropomyosin (36kD) Tpm4 Antibody (Monoclonal, TM228) catalog # MA1096. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Rabbit.

Product Info Summary

SKU: MA1096
Size: 100μg/vial
Reactive Species: Chicken, Human, Rabbit
Host: Mouse
Application: IHC, WB

Product Name

Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)

See all tropomyosin-4 products

SKU/Catalog Number

MA1096

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Tropomyosin (36kD) Tpm4 Antibody (Monoclonal, TM228) catalog # MA1096. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Rabbit.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1096)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

TM228

Isotype

Mouse IgG2a

Immunogen

Chicken gizzard tropomyosin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1096 is reactive to Tpm4 in Chicken, Human, Rabbit

Applications

MA1096 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, rabbit, -
Western blot, 0.5-1μg/ml, Human, chicken, rabbit

Validation Images & Assay Conditions

Gene/Protein Information For Tpm4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09495

Gene ID

24852

Gene Name

Tpm4

Full Name

Tropomyosin alpha-4 chain

Weight

28.51kDa

Superfamily

tropomyosin family

Alternative Names

TM30p1; tropomyosin 4; tropomyosin alpha-4 chain; tropomyosin-4 Tpm4|Tpm4.2.2cy, Tpm4|tropomyosin 4|tropomyosin alpha-4 chain|TM-4|tropomycin 4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tpm4, check out the Tpm4 Infographic

Tpm4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tpm4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for MA1096

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6 Customer Q&As for Anti-Tropomyosin(36kD) Tpm4 Antibody (Monoclonal, TM228)

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-14

Question

We are currently using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 for rabbit tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says chicken, human, rabbit. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

The anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) (MA1096) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-24

Question

Our lab want to know about to test anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 on chicken eye for research purposes, then I may be interested in using anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

The products we sell, including anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-11

Question

Would anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 work for WB with eye?

T. Jones

Verified customer

Asked: 2019-02-04

Answer

According to the expression profile of eye, TPM4 is highly expressed in eye. So, it is likely that anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) MA1096 will work for WB with eye.

Boster Scientific Support

Answered: 2019-02-04

Question

I have a question about product MA1096, anti-Tropomyosin(36kD) antibody (Monoclonal, TM228). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

E. Johnson

Verified customer

Asked: 2018-06-12

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-12

Question

Will MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Wu

Verified customer

Asked: 2017-08-28

Answer

You can see on the product datasheet, MA1096 anti-Tropomyosin(36kD) antibody (Monoclonal, TM228) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-08-28

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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MA1096
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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