|Validated Species:||Chicken, Human, Rat|
Data & Images
|Product Name||Anti-Tropomyosin(Sarcomeric) Antibody (Monoclonal, CH1)|
|Description||Mouse IgG monoclonal antibody for Tropomyosin (Sarcomeric), tropomyosin 1 (alpha) (TPM1) detection. Tested with WB, IHC-P, IHC-F in Human;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tropomyosin(Sarcomeric) Antibody (Monoclonal, CH1) CH1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1097)|
|Replacement Item||This antibody may replace the following items: sc-18174|sc-21806|sc-21807|sc-28543|sc-32514|sc-32516|sc-376339|sc-376541|sc-56603|sc-58868|sc-74480 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Chicken muscle tropomyosin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tropomyosin alpha-1 chain|
|Molecular Weight||32681 MW|
|Protein Function||Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.|
|Tissue Specificity||TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.|
|Subcellular Localization||Cytoplasm, cytoskeleton.|
|Alternative Names||Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1;Tpm1;Alpha-tm, Tpma;|