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Pack Size:100μg/vial
Validated Species:Chicken, Human, Rat
Clone Number: CH1
Application:IHC, WB
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Product Name Anti-Tropomyosin(Sarcomeric) Antibody (Monoclonal, CH1)
SKU/Catalog Number MA1097
Description Mouse IgG monoclonal antibody for Tropomyosin (Sarcomeric), tropomyosin 1 (alpha) (TPM1) detection. Tested with WB, IHC-P, IHC-F in Human;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Tropomyosin(Sarcomeric) Antibody (Monoclonal, CH1) CH1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1097)
Replacement Item This antibody may replace the following items: sc-18174|sc-21806|sc-21807|sc-28543|sc-32514|sc-32516|sc-376339|sc-376541|sc-56603|sc-58868|sc-74480 from Santa Cruz Biotechnology.
Host Mouse
Validated Species Chicken, Human, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen Chicken muscle tropomyosin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Monoclonal
Clone Number CH1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Tpm1
Protein Name Tropomyosin alpha-1 chain
Molecular Weight 32681 MW
Protein Function Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Tissue Specificity TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.
Subcellular Localization Cytoplasm, cytoskeleton.
Uniprot ID P04692
Alternative Names Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1;Tpm1;Alpha-tm, Tpma;
Research Areas TPM1|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tropomyosin alpha-1 chain

Tropomyosin is an alpha-helical, parallel, two-chain coiled coil which binds along the length of actin filaments in both muscle and non-muscle cells. This gene associates N-terminus to C-terminus to form a continuous strand along both sides of the actin filament and regulates its function. Tropomyosin contributes to most, if not all, functions of the actin cytoskeleton, and its role is essential for the viability of a wide range of organisms. The ability of tropomyosin to contribute to the many functions of the actin cytoskeleton is related to the temporal and spatial regulation of expression of tropomyosin isoforms.
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.