Anti-Tropomyosin(Sarcomeric) Antibody (Monoclonal, CH1)

SKU MA1097
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number CH1
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Tropomyosin(Sarcomeric) Antibody (Monoclonal, CH1)
SKU/Catalog Number MA1097
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Tropomyosin (Sarcomeric), tropomyosin 1 (alpha) (TPM1) detection. Tested with WB, IHC-P, IHC-F in Human;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Tropomyosin(Sarcomeric) Antibody (Monoclonal, CH1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1097)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Chicken muscle tropomyosin.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, rat, chicken, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, rat, chicken, -
Western blot, 1-2μg/ml, Human, rat, chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/m/a/ma1097 1.jpg

Figure 1. Western blot analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human A549 whole cell lysates,
Lane 8: human Caco-2 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Tropomyosin(Sarcomeric) antigen affinity purified monoclonal antibody (Catalog # MA1097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tropomyosin(Sarcomeric) at approximately 39KD. The expected band size for Tropomyosin(Sarcomeric) is at 33KD.

/m/a/ma1097 2.jpg

Figure 2. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097).
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

/m/a/ma1097 3.jpg

Figure 3. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097).
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

/m/a/ma1097 4.jpg

Figure 4. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097).
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

/m/a/ma1097 5.jpg

Figure 5. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097).
Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P04692
Gene Name TPM1
Protein Name Tropomyosin alpha-1 chain
Tissue Specificity TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.
Alternative Names Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1;Tpm1;Alpha-tm, Tpma;
Subcellular Localization Cytoplasm, cytoskeleton.
Molecular Weight 32681 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Research Areas Actin Etc, Cardiovascular, Cytoskeleton, Cytoskeleton / Ecm, Hypertrophy, Microfilaments, Signal Transduction

*You can search these to find other products in these research areas.
Background Tropomyosin is an alpha-helical, parallel, two-chain coiled coil which binds along the length of actin filaments in both muscle and non-muscle cells. This gene associates N-terminus to C-terminus to form a continuous strand along both sides of the actin filament and regulates its function. Tropomyosin contributes to most, if not all, functions of the actin cytoskeleton, and its role is essential for the viability of a wide range of organisms. The ability of tropomyosin to contribute to the many functions of the actin cytoskeleton is related to the temporal and spatial regulation of expression of tropomyosin isoforms.

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Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.