Product Info Summary
SKU: | P01720-1 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-Troponin I (cardiac) (Ser23/24) Antibody
SKU/Catalog Number
P01720-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Troponin I (cardiac) (Ser23/24) Antibody (Catalog # P01720-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Troponin I (cardiac) (Ser23/24) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01720-1)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
608
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 of mouse troponin 1, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Mouse.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01720-1 is reactive to TNNI3 in Human, Mouse, Rat
Applications
P01720-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
24.008kDa
Background of TNNI3
Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser-43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr-144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24 in the second lane (-). Phosphospecificity is shown in the first lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For TNNI3 (Source: Uniprot.org, NCBI)
Gene Name
TNNI3
Full Name
Troponin I, cardiac muscle
Weight
24.008kDa
Superfamily
troponin I family
Alternative Names
Cardiac troponin I; CMD1FF; CMD2A; CMH7MGC116817; cTnI; familial hypertrophic cardiomyopathy 7; RCM1; TNNC1troponin I, cardiac; troponin I type 3 (cardiac); troponin I, cardiac muscle TNNI3 CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI troponin I3, cardiac type troponin I, cardiac muscle|cardiomyopathy, dilated 2A (autosomal recessive)|troponin I type 3 (cardiac)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNNI3, check out the TNNI3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNNI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Troponin I (cardiac) (Ser23/24) Antibody (P01720-1)
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No publications found for P01720-1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Troponin I (cardiac) (Ser23/24) Antibody
Question
Does anti-Phospho-Troponin I (cardiac) (Ser23/24) antibody P01720-1 work on feline WB with heart muscle?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Our lab technicians have not validated anti-Phospho-Troponin I (cardiac) (Ser23/24) antibody P01720-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Phospho-Troponin I (cardiac) (Ser23/24) antibody P01720-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline heart muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-24
Question
I have attached the WB image, lot number and protocol we used for heart muscle using anti-Phospho-Troponin I (cardiac) (Ser23/24) antibody P01720-1. Please let me know if you require anything else.
P. Gonzalez
Verified customer
Asked: 2019-07-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-26
Question
I see that the anti-Phospho-Troponin I (cardiac) (Ser23/24) antibody P01720-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-01
Question
Is this P01720-1 anti-Phospho-Troponin I (cardiac) (Ser23/24) antibody reactive to the isotypes of TNNI3?
D. Dhar
Verified customer
Asked: 2017-06-09
Answer
The immunogen of P01720-1 anti-Phospho-Troponin I (cardiac) (Ser23/24) antibody is Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser23/24 of mouse troponin I, cardiac (cTnI). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-09