Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband™

TNNI2 antibody

Boster Bio Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband™ catalog # A07355-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07355-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, WB

Product Name

Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband™

View all TNNI2 Antibodies

SKU/Catalog Number

A07355-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband™ catalog # A07355-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07355-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Troponin I fast skeletal muscle/TNNI2 recombinant protein (Position: D3-S182).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07355-2 is reactive to TNNI2 in Human, Mouse, Rat

Applications

A07355-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

21.339kDa

Background of TNNI2

Troponin I, fast skeletal muscle is a protein that in humans is encoded by the TNNI2 gene. It is mapped to 11p15.5. This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25µg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml
Immunofluorescence, 5 µg/ml
Flow Cytometry, 1-3 μg/1x106 cells
Direct ELISA, 0.1-0.5µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For TNNI2 (Source: Uniprot.org, NCBI)

Gene Name

TNNI2

Full Name

Troponin I, fast skeletal muscle

Weight

21.339kDa

Superfamily

troponin I family

Alternative Names

skeletal, fast; troponin I type 2 (skeletal, fast); troponin I, fast skeletal muscle; Troponin I, fast-twitch isoform; troponin I, fast-twitch skeletal muscle isoform TNNI2 AMCD2B, DA2B, DA2B1, FSSV, fsTnI troponin I2, fast skeletal type troponin I, fast skeletal muscle|troponin I fast twitch 2|troponin I type 2 (skeletal, fast)|troponin I, skeletal, fast

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TNNI2, check out the TNNI2 Infographic

TNNI2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TNNI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband™

Question

We are currently using anti-Troponin I fast skeletal muscle/TNNI2 antibody A07355-2 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

The anti-Troponin I fast skeletal muscle/TNNI2 antibody (A07355-2) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-18

Order DetailsPrice
A07355-2

100μg

$370
A07355-2-10ug

10μg sample (liquid)

$99
A07355-2-Biotin

100 μg Biotin conjugated

$570
A07355-2-Cy3

100 μg Cy3 conjugated

$570
A07355-2-Dylight488

100 μg Dylight488 conjugated

$570
A07355-2-Dylight550

100 μg Dylight550 conjugated

$570
A07355-2-Dylight594

100 μg Dylight594 conjugated

$570
A07355-2-FITC

100 μg FITC conjugated

$570
A07355-2-HRP

100 μg HRP conjugated

$570
A07355-2-APC

100 μg APC conjugated

$670
A07355-2-PE

100 μg PE conjugated

$670
A07355-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A07355-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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