| SKU: | MA1098 |
|---|---|
| Pack Size: | 100μg/vial |
| Validated Species: | Chicken, Rabbit, Rat |
| Clonality: | Monoclonal |
| Clone Number: | JLT-12 |
| Host: | Mouse |
| Application: | IHC, WB |
- Technical
Specs - Kit
Components - Gene
Information - Application
Data & Images - Reviews
Publications - Customer
Q&As
Overview
| Product Name | Anti-Troponin T Antibody (Monoclonal, JLT-12) |
|---|---|
| SKU/Catalog Number | MA1098 |
| Description | Mouse IgG monoclonal antibody for Troponin T, troponin T type 1 (skeletal, slow); troponin T type 2 (cardiac); troponin T type 3 (skeletal, fast) (TNNT1; TNNT2; TNNT3) detection. Tested with WB, IHC-P, IHC-F in Rat;rabbit;chicken. No cross reactivity with other proteins. |
| Cite This Product | Anti-Troponin T Antibody (Monoclonal, JLT-12) JLT-12 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1098) |
| Replacement Item | This antibody may replace the following items: sc-162313|sc-162316|sc-162318|sc-162319|sc-162317|sc-162314|sc-162311 from Santa Cruz Biotechnology. |
| Host | Mouse |
| Validated Species | Chicken, Rabbit, Rat |
| Application | IHC, WB *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Recommended Detection Systems | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $50. Contact us for more information **Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018! |
| Immunogen | Troponin T from rabbit skeletal muscle. |
| Cross Reactivity | No cross reactivity with other proteins |
| Pack Size | 100μg/vial |
Properties
| Clonality | Monoclonal |
|---|---|
| Clone Number | JLT-12 |
| Form | Lyophilized |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Concentration | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Purification | Ascites |
| Isotype | Mouse IgG1 |
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | Tnnt3 |
|---|---|
| Protein Name | Troponin T, slow skeletal muscle; Troponin T, cardiac muscle; Troponin T, fast skeletal muscle |
| Molecular Weight | 30750 MW |
| Protein Function | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. . |
| Sequence Similarities | Belongs to the troponin T family. |
| Uniprot ID | P09739 |
| Alternative Names | Troponin T, fast skeletal muscle;TnTf;Fast skeletal muscle troponin T;fTnT;Tnnt3; |
| Research Areas | signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|troponin| |
Background for Troponin T, slow skeletal muscle; Troponin T, cardiac muscle; Troponin T, fast skeletal muscle
The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle(TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle(TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.
Related Products
Goat Anti-Mouse IgG Secondary Antibody, HRP Conjugate (BA1054)
Price: FREE if purchase with Primary antibody
Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001)
Price: $100
SuperVision IHC Kit with anti-Mouse IgG (SV0001-1)
Price: $150
Anti-Troponin T Antibody (Monoclonal, JLT-12) Images
Click the images to enlarge.
Anti-Troponin T antibody, MA1098, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
IHC(P): Rat Skeletal Muscle Tissue
Publications
Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tnnt2 antibody, troponin t antibody
