Anti-Troponin T Antibody (Monoclonal, JLT-12)

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Datasheet

Protocols

COA

MSDS

SKU	MA1098
Size	100μg/vial
Reactivity	Chicken, Rabbit, Rat
Clonality	Monoclonal
Clone Number	JLT-12
Host	Mouse
Ig Isotype	Mouse IgG1
Applications	IHC, WB

• Overview
• Assay Details
• Target Info
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Overview

Product Name	Anti-Troponin T Antibody (Monoclonal, JLT-12)
SKU/Catalog Number	MA1098
Storage & Handling	At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size	100μg/vial
Description	Mouse IgG monoclonal antibody for Troponin T, troponin T type 1 (skeletal, slow); troponin T type 2 (cardiac); troponin T type 3 (skeletal, fast) (TNNT1; TNNT2; TNNT3) detection. Tested with WB, IHC-P, IHC-F in Rat;rabbit;chicken. No cross reactivity with other proteins.
Cite This Product	Anti-Troponin T Antibody (Monoclonal, JLT-12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1098)
Host	Mouse
Contents/Buffer	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form	Lyophilized
Immunogen	Troponin T from rabbit skeletal muscle.
Reactivity	Chicken, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Rat, rabbit, chicken, By Heat
Immunohistochemistry(Frozen Section), 4-8μg/ml, Rat, rabbit, chicken, -
Western blot, 2-4μg/ml, Rat, rabbit, chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

Anti-Troponin T antibody, MA1098, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id	P09739
Gene Name	Tnnt3
Protein Name	Troponin T, fast skeletal muscle
Alternative Names	Troponin T, fast skeletal muscle;TnTf;Fast skeletal muscle troponin T;fTnT;Tnnt3;
Molecular Weight	30750 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function	Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. .
Research Areas	Chicken, Rabbit, Rat *You can search these to find other products in these research areas.
Background	The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle(TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle(TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.

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Monoclonal clone# G2 antibody for TNNT1; TNNT2; TNNT3 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Chicken. TNNT1; TNNT2; TNNT3 information: Molecular Weight: 30750 MW; .

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MA1098

Promotion :

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$280.00

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Troubleshooting

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Customer Q&As

• Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
  A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
• Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
  A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
• Q: Can you provide monoclonal antibodies carrier free?
  A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
• Q: What are some alternative names that could be used to describe this product?
  A: Some common names include but are not limited to tnnt2 antibody, troponin t antibody