Anti-Troponin T Antibody (Monoclonal, JLT-12)

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SKU MA1098
Size 100μg/vial
Reactivity Chicken, Rabbit, Rat
Clonality Monoclonal
Clone Number JLT-12
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Troponin T Antibody (Monoclonal, JLT-12)
SKU/Catalog Number MA1098
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Troponin T, troponin T type 1 (skeletal, slow); troponin T type 2 (cardiac); troponin T type 3 (skeletal, fast) (TNNT1; TNNT2; TNNT3) detection. Tested with WB, IHC-P, IHC-F in Rat;rabbit;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Troponin T Antibody (Monoclonal, JLT-12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1098)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Troponin T from rabbit skeletal muscle.
Reactivity Chicken, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Rat, rabbit, chicken, By Heat
Immunohistochemistry(Frozen Section), 4-8μg/ml, Rat, rabbit, chicken, -
Western blot, 2-4μg/ml, Rat, rabbit, chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1098 1 IHC anti troponin t antibody monoclonal jlt 12.jpg

Anti-Troponin T antibody, MA1098, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P09739
Gene Name TNNT1; TNNT2; TNNT3
Protein Name Troponin T, fast skeletal muscle
Alternative Names Troponin T, fast skeletal muscle;TnTf;Fast skeletal muscle troponin T;fTnT;Tnnt3;
Molecular Weight 30750 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. .
Research Areas Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction

*You can search these to find other products in these research areas.
Background The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle(TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle(TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.

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Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

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Troubleshooting

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Publications

Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...
PubMed ID21476855

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tnnt2 antibody, troponin t antibody