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| SKU | MA1098 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Chicken, Rabbit, Rat |
| Clonality | Monoclonal |
| Clone Number | JLT-12 |
| Host | Mouse |
| Ig Isotype | Mouse IgG1 |
| Applications | IHC, WB |
Overview
| Product Name | Anti-Troponin T Antibody (Monoclonal, JLT-12) |
|---|---|
| SKU/Catalog Number | MA1098 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for Troponin T, troponin T type 1 (skeletal, slow); troponin T type 2 (cardiac); troponin T type 3 (skeletal, fast) (TNNT1; TNNT2; TNNT3) detection. Tested with WB, IHC-P, IHC-F in Rat;rabbit;chicken. No cross reactivity with other proteins. |
| Cite This Product | Anti-Troponin T Antibody (Monoclonal, JLT-12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1098) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Immunogen | Troponin T from rabbit skeletal muscle. |
| Reactivity | Chicken, Rabbit, Rat |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Rat, rabbit, chicken, By Heat
Immunohistochemistry(Frozen Section), 4-8μg/ml, Rat, rabbit, chicken, -
Western blot, 2-4μg/ml, Rat, rabbit, chicken
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Rat, rabbit, chicken, By Heat
Immunohistochemistry(Frozen Section), 4-8μg/ml, Rat, rabbit, chicken, -
Western blot, 2-4μg/ml, Rat, rabbit, chicken
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).Images And Assay Conditions
Anti-Troponin T antibody, MA1098, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P09739 |
|---|---|
| Gene Name | TNNT1; TNNT2; TNNT3 |
| Protein Name | Troponin T, fast skeletal muscle |
| Alternative Names | Troponin T, fast skeletal muscle;TnTf;Fast skeletal muscle troponin T;fTnT;Tnnt3; |
| Molecular Weight | 30750 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. . |
|---|---|
| Research Areas | Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction *You can search these to find other products in these research areas. |
| Background | The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle(TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle(TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively. |
Other Recommended Resources
Here are featured tools and databases that you might find useful.
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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.Troubleshooting
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Publications
Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, Peng X, Li W, Xu Z, Sun L, Xu W. Stem Cells Dev. 2012 Jan;21(1):67-75. Doi: 10.1089/Scd.2010.0519. Epub 2011 Jun 1. 5-Azacytidine Induces Cardiac Differentiation Of Human Umbilical Cord-Derived Mesenchy...
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tnnt2 antibody, troponin t antibody