Anti-TRPC5 Antibody Picoband™

TRPC5 antibody

Boster Bio Anti-TRPC5 Antibody Picoband™ catalog # PB9271. Tested in WB applications. This antibody reacts with Human. Independently reviewed in 1 review(s).

Product Info Summary

SKU: PB9271
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-TRPC5 Antibody Picoband™

View all TRPC5 Antibodies

SKU/Catalog Number

PB9271

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRPC5 Antibody Picoband™ catalog # PB9271. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRPC5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9271)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRPC5 recombinant protein (Position: K684-L973). Human TRPC5 shares 90% amino acid (aa) sequence identity with mouse TRPC5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9271 is reactive to TRPC5 in Human

Applications

PB9271 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

111 kDa

Calculated molecular weight

111.412kDa

Background of TRPC5

Short transient receptor potential channel 5 (TrpC5), also known as TRP-5, is a protein that in humans is encoded by the TRPC5 gene. TRPC5 is subtype of the TRPC family of mammalian transient receptor potential ion channels. It is mapped to Xq23. The predicted 973-amino acid TRPC5 protein has a calculated molecular mass of 111.5 kD. It contains the characteristic 8 predicted transmembrane domains (TM1 through TM8), including a pore region (TM7) between TM6 and TM8. TRPC5 is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel, and it is a candidate for the regulation of calcium waves. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For TRPC5 (Source: Uniprot.org, NCBI)

Gene Name

TRPC5

Full Name

Short transient receptor potential channel 5

Weight

111.412kDa

Superfamily

transient receptor (TC 1.A.4) family

Alternative Names

hTRP5; hTRP-5; short transient receptor potential channel 5; transient receptor potential cation channel, subfamily C, member 5; Transient receptor protein 5; TRP-5; TRP5transient receptor potential channel 5; TrpC5 TRPC5 PPP1R159, TRP5 transient receptor potential cation channel subfamily C member 5 short transient receptor potential channel 5|TRP-5|hTRP5|protein phosphatase 1, regulatory subunit 159|transient receptor potential channel 5|transient receptor protein 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRPC5, check out the TRPC5 Infographic

TRPC5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRPC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Reviews For Anti-TRPC5 Antibody Picoband™

0

Anti-TRPC5 Tried in Human Tissue Sample--Saikat Chakraborty, Psychiatry / Neuroscience, Northwestern University Feinberg School of Medicine, Research Scientist

Excellent

Source: Biocompare.com

Application Western Blot
Sample Human Tissue
Detection Fluorescent based

"We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 antibody worked perfectly in detection in human tissue. Perfect band obtained at desired molecular weight. No extra bands. I recommend."

Verified customer

Submitted

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4 Customer Q&As for Anti-TRPC5 Antibody Picoband™

Question

See below the WB image, lot number and protocol we used for frontal cortex using anti-TRPC5 antibody PB9271. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-23

Question

We are currently using anti-TRPC5 antibody PB9271 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

The anti-TRPC5 antibody (PB9271) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-04

Question

Does PB9271 anti-TRPC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-26

Answer

You can see on the product datasheet, PB9271 anti-TRPC5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-26

Question

Will anti-TRPC5 antibody PB9271 work on monkey WB with fetal brain?

K. Taylor

Verified customer

Asked: 2017-05-25

Answer

Our lab technicians have not tested anti-TRPC5 antibody PB9271 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-TRPC5 antibody PB9271 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey fetal brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-25

Order DetailsPrice
PB9271

100μg

$370
PB9271-10ug

10μg sample (liquid)

$99
PB9271-Biotin

100 μg Biotin conjugated

$570
PB9271-Cy3

100 μg Cy3 conjugated

$570
PB9271-Dylight488

100 μg Dylight488 conjugated

$570
PB9271-Dylight550

100 μg Dylight550 conjugated

$570
PB9271-Dylight594

100 μg Dylight594 conjugated

$570
PB9271-FITC

100 μg FITC conjugated

$570
PB9271-HRP

100 μg HRP conjugated

$570
PB9271-APC

100 μg APC conjugated

$670
PB9271-PE

100 μg PE conjugated

$670
PB9271-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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