Data & Images
|Product Name||Anti-TRPC6 Antibody|
|Description||Rabbit IgG polyclonal antibody for Short transient receptor potential channel 6(TRPC6) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-TRPC6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1754)|
|Replacement Item||This antibody may replace the following items: sc-19196|sc-19197 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human TRPC6(249-265aa HDYFCKCNDCNQKQKHD), different from the related rat and mouse sequences by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Short transient receptor potential channel 6(TrpC6)|
|Molecular Weight||106326 MW|
|Protein Function||Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.|
|Tissue Specificity||Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. .|
|Sequence Similarities||Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.|
|Subcellular Localization||Membrane ; Multi-pass membrane protein .|
|Alternative Names||Short transient receptor potential channel 6;TrpC6;Transient receptor protein 6;TRP-6;TRPC6;TRP6;|
|Research Areas|||signal transduction|signaling pathway|calcium signaling|calcium channels| signal transduction||
Background for Short transient receptor potential channel 6(TrpC6)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TRPC6 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TRPC6 Antibody Images
Click the images to enlarge.
Lane 1: Rat Lung Tissue Lysate
Lane 2: 293T Cell Lysate
Lane 3: 293T Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Rat Brain Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,