|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-TRPM2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Transient receptor potential cation channel subfamily M member 2(TRPM2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-TRPM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2231)|
|Replacement Item||This antibody may replace the following items: sc-19198|sc-368395 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human TRPM2(91-106aa CGYTHEQHLEEATKPH), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Transient receptor potential cation channel subfamily M member 2|
|Molecular Weight||171198 MW|
|Protein Function||Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity. .|
|Tissue Specificity||Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes. .|
|Sequence Similarities||Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.|
|Subcellular Localization||Membrane ; Multi-pass membrane protein .|
|Alternative Names||Transient receptor potential cation channel subfamily M member 2;220.127.116.11;Estrogen-responsive element-associated gene 1 protein;Long transient receptor potential channel 2;LTrpC-2;LTrpC2;Transient receptor potential channel 7;TrpC7;TRPM2;EREG1, KNP3, LTRPC2, TRPC7;|
|Research Areas|||signal transduction|signaling pathway|calcium signaling|calcium channels| signal transduction| cell biology|other antibodies|oxidative stress| metabolism|pathways and processes|redox metabolism||
Background for Transient receptor potential cation channel subfamily M member 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TRPM2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TRPM2 Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: Mouse Liver Tissue Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: A549 Cell Lysate
IHC(P): Human Tonsil Tissue
IHC(P): Rat Brain Tissue
IHC(F): Rat Spleen Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,