Product Info Summary
SKU: | M00789 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TRPM7 Monoclonal Antibody
SKU/Catalog Number
M00789
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRPM7 Monoclonal Antibody catalog # M00789. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPM7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00789)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADGD-20
Isotype
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Immunogen
A synthesized peptide derived from human TRPM7
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00789 is reactive to TRPM7 in Human, Mouse, Rat
Applications
M00789 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
150 kDa-200 kDa
Calculated molecular weight
212.697kDa
Background of TRPM7
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TRPM7 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TRPM7 (Source: Uniprot.org, NCBI)
Gene Name
TRPM7
Full Name
Transient receptor potential cation channel subfamily M member 7
Weight
212.697kDa
Alternative Names
CHAK; CHAK1FLJ25718; Channel-kinase 1; EC 2.7.11.1; Long transient receptor potential channel 7; LTRPC ion channel family member 7; LTrpC7; LTrpC-7; LTRPC7FLJ20117; transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential-phospholipase C-interacting kinase; TRP-PLIK TRPM7 ALSPDC, CHAK, CHAK1, LTRPC7, LTrpC-7, TRP-PLIK transient receptor potential cation channel subfamily M member 7 transient receptor potential cation channel subfamily M member 7|LTRPC ion channel family member 7|channel-kinase 1|long transient receptor potential channel 7|transient receptor potential-phospholipase C-interacting kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRPM7, check out the TRPM7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPM7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRPM7 Monoclonal Antibody (M00789)
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No publications found for M00789
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Recommended Resources
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TRPM7 Monoclonal Antibody
Question
Is a blocking peptide available for product anti-TRPM7 Monoclonal antibody (M00789)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We do provide the blocking peptide for product anti-TRPM7 Monoclonal antibody (M00789). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-07
Question
Will anti-TRPM7 Monoclonal antibody M00789 work on horse WB with colon placenta?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
Our lab technicians have not tested anti-TRPM7 Monoclonal antibody M00789 on horse. You can run a BLAST between horse and the immunogen sequence of anti-TRPM7 Monoclonal antibody M00789 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse colon placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-30
Question
Will anti-TRPM7 Monoclonal antibody M00789 work for IF with liver?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of liver, TRPM7 is highly expressed in liver. So, it is likely that anti-TRPM7 Monoclonal antibody M00789 will work for IF with liver.
Boster Scientific Support
Answered: 2019-08-20
Question
I was wanting to use your anti-TRPM7 Monoclonal antibody for IF for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
As indicated on the product datasheet, M00789 anti-TRPM7 Monoclonal antibody has been validated for Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-19
Question
I have a question about product M00789, anti-TRPM7 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Mitchell
Verified customer
Asked: 2016-06-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00789 anti-TRPM7 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-24
Question
Is this M00789 anti-TRPM7 Monoclonal antibody reactive to the isotypes of TRPM7?
S. Brown
Verified customer
Asked: 2013-10-28
Answer
The immunogen of M00789 anti-TRPM7 Monoclonal antibody is A synthesized peptide derived from human TRPM7. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-28