Data & Images
|Product Name||Anti-TRPV4 Antibody|
|Description||Rabbit IgG polyclonal antibody for Transient receptor potential cation channel subfamily V member 4(TRPV4) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-TRPV4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1979)|
|Replacement Item||This antibody may replace the following items: sc-47527|sc-98592|sc-16485|sc-47525 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human TRPV4(113-133aa YRHHSSDNKRWRKKIIEKQPQ), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Transient receptor potential cation channel subfamily V member 4|
|Molecular Weight||98281 MW|
|Protein Function||Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. .|
|Tissue Specificity||Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level). .|
|Sequence Similarities||Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Cell junction, adherens junction . Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. .|
|Alternative Names||Transient receptor potential cation channel subfamily V member 4;TrpV4;Osm-9-like TRP channel 4;OTRPC4;Transient receptor potential protein 12;TRP12;Vanilloid receptor-like channel 2;Vanilloid receptor-like protein 2;VRL-2;Vanilloid receptor-related osmotically-activated channel;VR-OAC;TRPV4;VRL2, VROAC;|
|Research Areas|||neuroscience|neurotransmission|receptors / channels|more ion channels| neuroscience|sensory system|somatosensory system|nociception||
Background for Transient receptor potential cation channel subfamily V member 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TRPV4 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TRPV4 Antibody Images
Click the images to enlarge.
Lane 1: Rat Kidney Tissue Lysate
Lane 2: HEPA Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,