Product Info Summary
SKU: | A00565-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TRPV4 Antibody Picoband™
SKU/Catalog Number
A00565-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPV4 Antibody Picoband™ catalog # A00565-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPV4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00565-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPV4 recombinant protein (Position: N66-L871).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00565-2 is reactive to TRPV4 in Human, Mouse, Rat
Applications
A00565-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
98 kDa
Calculated molecular weight
98.281kDa
Background of TRPV4
TRPV4 (Transient Receptor Potential Cation Channel Subfamily V Member 4), also known as TRP12, OTRPC4 or VROAC, is a protein that in humans is encoded by the TRPV4 gene. This gene encodes TRPV4, a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. By genomic sequence analysis, Liedtke et al. (2000) mapped the TRPV4 gene to chromosome 12q24.1. They mapped the mouse Trpv4 gene to distal chromosome 5 by radiation hybrid analysis. Wissenbach et al. (2000) found that hypoosmotic conditions rapidly activated TRP12, while hyperosmotic conditions inhibited the activity.Strotmann et al. (2000) showed that OTRPC4 was responsive to changes in extracellular osmolarity in the physiologically relevant range and was expressed in tissues exposed to changing osmotic environments.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TRPV4 using anti-TRPV4 antibody (A00565-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV4 antigen affinity purified polyclonal antibody (Catalog # A00565-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV4 at approximately 98KD. The expected band size for TRPV4 is at 98KD.
Protein Target Info & Infographic
Gene/Protein Information For TRPV4 (Source: Uniprot.org, NCBI)
Gene Name
TRPV4
Full Name
Transient receptor potential cation channel subfamily V member 4
Weight
98.281kDa
Superfamily
transient receptor (TC 1.A.4) family
Alternative Names
CMT2Cosmosensitive transient receptor potential channel 4; HMSN2C; Osm-9-like TRP channel 4; OTRPC4SSQTL1; transient receptor potential cation channel subfamily V member 4; transient receptor potential cation channel, subfamily V, member 4; Transient receptor potential protein 12; TRP12OSM9-like transient receptor potential channel 4; TrpV4; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; vanilloid receptor-related osmotically activated channel; Vanilloid receptor-related osmotically-activated channel; VRL2; VRL-2; VR-OAC; VROACSPSMA TRPV4 BCYM3, CMT2C, HMSN2C, OTRPC4, SMAL, SPSMA, SSQTL1, TRP12, VRL2, VROAC transient receptor potential cation channel subfamily V member 4 transient receptor potential cation channel subfamily V member 4|OSM9-like transient receptor potential channel 4|osm-9-like TRP channel 4|osmosensitive transient receptor potential channel 4|transient receptor potential protein 12|vanilloid receptor-like channel 2|vanilloid receptor-related osmotically activated channel
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRPV4, check out the TRPV4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRPV4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRPV4 Antibody Picoband™ (A00565-2)
Hello CJ!
No publications found for A00565-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TRPV4 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-TRPV4 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question