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SKU:MA1099
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:WH-3
Application:WB
Price: $200.00
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Overview

Product Name Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)
SKU/Catalog Number MA1099
Description Mouse IgG monoclonal antibody for Tryptophan Hydroxylase, tryptophan hydroxylase 1; tryptophan hydroxylase 2 (TPH1; TPH2) detection. Tested with WB in Human;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) WH-3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1099)
Replacement Item This antibody may replace the following items: sc-161446|sc-161445 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG3
Validated Species Human, Rabbit, Rat
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant rabbit tryptophan hydroxylase.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number WH-3
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG3

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Tph2
Protein Name Tryptophan 5-hydroxylase 1; Tryptophan 5-hydroxylase 2
Molecular Weight 55621 MW
Tissue Specificity TPH1: Isoform 2 seems to be less widely expressedthan isoform 1.
Sequence Similarities Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Uniprot ID Q8CGU9
Alternative Names Tryptophan 5-hydroxylase 2;1.14.16.4;Neuronal tryptophan hydroxylase;Tryptophan 5-monooxygenase 2;Tph2;Ntph;
Research Areas neuroscience|neurotransmitter|biogenic amines|serotonin / 5ht|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tryptophan 5-hydroxylase 1; Tryptophan 5-hydroxylase 2

Tryptophan hydroxylase is the rate-limiting enzyme in the synthesis of serotonin(5-hydroxytryptamine, or 5HT). Tryptophan hydroxylase\atalyzes the biopterin-dependent monooxygenation of tryptophan to5-hydroxytryptophan(5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Human tryptophan hydroxylase(TPH) is mapped to chromosome 11p15.3-p14 by in situ hybridization.

Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) Images

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Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)
Anti-Tryptophan Hydroxylase antibody (monoclonal), MA1099, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate

Customer Reviews

Review by
Rating:

Source: Biocompare.com

"Outstanding signal of cell bodies and processes"

--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")

Applications Immunohistochemistry
Sample Wistar rat brain
Detection Fluorescence

"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."

(Posted on 8/23/2016)
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tph antibody, tph1 antibody