Data & Images
|Product Name||Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)|
|Description||Mouse IgG monoclonal antibody for Tryptophan Hydroxylase, tryptophan hydroxylase 1; tryptophan hydroxylase 2 (TPH1; TPH2) detection. Tested with WB in Human;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) WH-3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1099)|
|Replacement Item||This antibody may replace the following items: sc-161446|sc-161445 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rabbit, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant rabbit tryptophan hydroxylase.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tryptophan 5-hydroxylase 1; Tryptophan 5-hydroxylase 2|
|Molecular Weight||55621 MW|
|Tissue Specificity||TPH1: Isoform 2 seems to be less widely expressedthan isoform 1.|
|Sequence Similarities||Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.|
|Alternative Names||Tryptophan 5-hydroxylase 2;22.214.171.124;Neuronal tryptophan hydroxylase;Tryptophan 5-monooxygenase 2;Tph2;Ntph;|
|Research Areas||neuroscience|neurotransmitter|biogenic amines|serotonin / 5ht||
Background for Tryptophan 5-hydroxylase 1; Tryptophan 5-hydroxylase 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.5-1μg/ml, Human, rat, rabbit|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
- Review by Unattributed Review*
"Outstanding signal of cell bodies and processes"
--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")
Applications Immunohistochemistry Sample Wistar rat brain Detection Fluorescence
"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."